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Troubleshooting Guides - Gas Production System (after July 2009)

Instrument Troubleshooting

(Modules will have red caps)

Q1. How can I be sure that the ANKOM Gas Production System is functioning?

Q2. How do I know when a module is functioning properly?

Q3. Why does cumulative pressure in a module decrease or go negative?

Q4. My module doesn't seem to be holding pressure. What should I do?

Q5. How often should I change the gasket seal between the electronics module and the glass bottle?

Q6. Do I need to replace the membrane on the septa port after I insert a syringe?

Q7. My module doesn't seem to be releasing pressure at its set level. What should i do?

Q8. How do I clean my vent valve?

Q9. How long does the valve stay open when I click the "valve open" check box in the GPM software?

Q10. Where is the data from the study stored?

Q11. Can current data be retrieved from a run that has been interrupted do to power outages, computer glitches, etc?

Q12. What is a Network?

Q13. What do different colored cells on my GPM software screen represent?

Q14. What is the Module's battery life?

Q15. What can I do to preserve battery life?

Q16. When should I recharge my rechargeable battery pack (RF16)?

Q17. How long does it take to recharge my batteries?

Q18. Can I recharge my alkaline battery pack (RF26)?

Q19. Should my battery pack get hot?

Q20. Should the solenoid cylinder in the module get hot?

Q21. Why do my modules read different temperatures even though they are in the same environment?

Q22. Why do some of my modules show a temperature value and some don't?

Q23. Why are my modules with temperature sensors not communicating with the computer?

Q24. Can I save the graphs that are automatically generated on the GPM Screen?

Q25. Why am I getting "error saving to xls file" messages now that I've set up my new GPM System?

Q1. How can I be sure that the ANKOMRF Gas Production System is functioning properly and producing proper results?

If you observe results that you don't understand and you want to verify that the ANKOMRF Gas Production System is functioning properly, run the RF Validation Test detailed in Service Procedure 124

Q2. How do I know when a module is functioning properly?

See Service Procedure 171.

Q3. Why does the cumulative pressure in a module decrease or go negative?

Refer to the RF Curve Identification Chart in Service Procedure 184 for curve illustrations.
The cumulative pressure in a module could decrease or go negative for the following reasons:

1) Communication errors between modules and the coordinator

-
The module loses communications with the coordinator at the time of a valve vent cycle. When the module re-establishes communication, the actual pressure may be lower than what it was before the communication loss. This will be recorded as a cumulative pressure drop. The cumulative curve can be adjusted by adding an offset from the point of pressure loss. This offset is the difference between the pressure just before the communication loss and the pressure immediately following the communication loss.

2) Permeability through elastomeric components
- For robust performance, the ANKOMRF Gas Production System uses elastomeric components in the valve assembly. These components are slightly CO2 permeable. In a pure CO2 environment under 2 psi pressure, studies show that the permeability rate is approximately 0.02 psi/hr. As a result, this phenomenon can cause a slight loss of pressure that is evident at the end of the study when gas production is leveling off. This is a normal occurrence that can be corrected by running a blank in your study. See the RF Getting Started Guide for more information.

3) Leak in the system

-A faulty seal between the electronics module and the bottle can cause the system to leak. Before running a study, inspect the rim of the bottle and remove any loose debris. Clean the gasket on the underside of the module, or replace if damaged. To ensure that the seal is good, attach the module to the bottle by screwing it on finger tight plus an additional 1/8 turn. Execute Service Procedure 171 to test for leaks.

-To ensure that the vent valve is clean, see Service Procedure 172.

-For bottles with Septa Ports, ensure that the septa connection is tight and the septum does not have holes.

4)
Temperature change

-Pressure can change when temperature changes. For instance, if your modules are in an oven and the oven door opens, the pressure in the modules will decrease.

5) Absorption of CO2 in the liquid

-In the beginning of a study, CO2 can dissolve into the fluid until the fluid becomes CO2 saturated. If CO2 absorption happens faster than CO2 production, then the net result will be negative

My module doesn't seem to be holding pressure. What should I do?

See Service Procedure 171.

Q5. How often should I change the gasket seal between the electronics module and the glass bottle?

You should replace the gasket whenever it appears damaged or loose. If you open and close the bottles once a week (on average), you should replace the gasket once every six months.

Q6. Do I need to replace the membrane on the septa port after it is pierced it with a syringe?

No. This membrane material will self-seal after being pierced . However, it is important to use a small gauge needle and to avoid puncturing the membrane in the same location. In general you should replace the membrane after 12 insertions or when leaking. When re-sealing the septa port, only tighten the plastic fitting to finger tight.

Q7. My module doesn't seem to be releasing pressure at its set level. What should I do?

See Service Procedure 171.

Q8. How do I clean my vent valve?

See Service Procedure 172.

Q9. How long does the valve stay open when I click the "valve open" check box in the GPM software?

The valve will stay open for 3 minutes. The valve(s) can be closed before this time by clicking the "valve open" check box (to remove the check mark from the box) , clicking the "close valves" button, or by clicking the "Record" button. Since opening the valves moderately drains the batteries, it is recommended
that they only be opened as needed.

Q10. Where is the data from the study stored?

When you press the Record button on your GPM software screen, the program automatically stores your data in the file shown in the AutoSave location box. We recommend that you create a new file name before each study to make sure that previous data is not overwritten. If you do not change this file name, the program will store the study date in C:\ Program Files\ ANKOM\Gas Pressure Monitor\GPM AutoSave.xls.

Q11. Can current data be retrieved from a run that has been interrupted do to power outages, computer glitches, etc.?

When you press the Record button on your GPM software screen, the program automatically stores your data in the file shown in the AutoSave location box. Therefore, if you suffer a computer interruption during a study, you will be able to retrieve all of the data from the beginning of the study until the time of the computer interruption. To avoid the possibility of overwriting the data, do not start a new study until you retrieve the data from the interrupted study.

We recommend that you create a new file name before each study to make sure that previous data is not overwritten. If you do not change this file name, the program will store the study data in C:\Program Files\ANKOM\Gas Pressure Monitor\GPM AutoSave.xls.

Q12. What is a Network?

A Network consists of 1-PC, 1-RF2-Base Coordinator, and 1-RF5 Reference (measures ambient pressure). Each network can handle up to 50-RF1 Modules.

When there are more than 50-RF1 Modules, a new network is required. A given location can have up to 5 Networks, with up to 250 RF- Modules.

Q13. What do the different colored cells on my GPM software screen represent?

See the Instrument Software Functionality section of the ANKOMRF Gas Production System Operator's Manual.

Q14. What is the Module's battery life?

The RF1 modules can be powered by a rechargeable battery pack (RF16) or an alkaline battery pack (RF26)

Rechargeable Batteries

Rechargeable batteries lose charge when not being used. Always charge your rechargeable battery packs for at least 3 hours using the ANKOM Battery Charger (part # RF25) just prior to their use. A fully charged battery when plugged into a module should read 6.7V or higher on the GPM software screen. Typically, a fully charged rechargeable battery pack should be able to power a module for at least 10 days under normal conditions (20-40C, GPM settings of 1 minute Live Interval and 1 Valve Open sequence per hour).

Individual performance may vary depending on testing conditions. Although modules will function properly, rechargeable battery packs may have to be recharged at more frequent intervals when operating at lower temperatures. When running the system, remember to check the battery voltage daily and change the battery pack when the voltage decreases to 6.3V or lower (the GPM software will show a YELLOW color under these conditions.) Changing the battery pack during the run will not affect the results.

Alkaline Batteries

For the alkaline battery packs, the chart below shows the battery life expectancy with three different Live Intervals set within the GPM software.

Live Interval

Battery Life

1 second

50 hrs

10 seconds

500 hrs

60 seconds

3,000 hrs


Q15. What can I do to preserve battery life?

Always unplug the battery pack when a module is not being used. Utilize longer Live Interval and Recording Interval times during testing.

Q16. When should I recharge my rechargeable battery packs (RF16)?

Rechargeable batteries lose charge when not being used. Always charge your rechargeable battery packs for at least 3 hours using the ANKOM Batter Charger (part #RF25) just prior to their use. A fully charged battery when plugged into a module should read 6.7V or higher on the GPM software screen. Typically, a fully charged rechargeable battery pack should be able to power a module for at least 10 days under normal conditions (20-40C,GPM settings of 1 minute Live Interval and 1 Valve Open sequence per hour.)

Individual performance may vary depending on testing conditions. Although modules will function properly, rechargeable battery packs may have to be recharged at more frequent intervals when operating at lower temperatures. When running the system, remember to check the battery voltage daily and change the battery pack when the voltage decreases to 6.3V or lower (the GPM software will show a YELLOW color under these conditions.) Changing the battery pack during the run will now affect the results.

Q17. How long does it take to recharge my batteries?

Typically it takes three hours to fully charge a battery pack. Please note that if you are using a single station charger, you must cycle power to the charger every time you reconnect a battery pack. You do not have to cycle power when using the 10- Station Battery Charger.

Q18. Can I recharge my alkaline battery pack (RF26)?

No. Attempts to recharge may cause rupture or leaking of hazardous liquids that will corrode the equipment.

Q19. Should my battery pack get hot?

No. If this happens, the battery pack may be defective or the module's circuit board may have failed. In the module, install another fully charged battery pack. Then run the module functional test found in Service Procedure 171. If this test fails or the battery pack gets hot, then replace the module.


Q20. Should the solenoid cylinder in the module get hot?

The solenoid cylinder in the module is part of the valve assembly. If the "valve open" check box is checked (in the GPM software), then the valve is continuously open. In this case, the solenoid will get hot. During a recording, the solenoid will not get hot.

Q21. Why do my modules read different temperatures even though they are in the same environment?

The sensors are accurate to within +/-2C
over a range of 5C-60C. To account for any Module-to-Module differences in temperature sensing capability, the GPM software provides a calibration screen that can be used before data recording begins. See the Instrument Software Functionality section of the ANKOM Gas Production System Operator's Manual for details about calibrating the temperature sensors.

Q22. Why do some of my modules show a temperature value and some don't?

A module that shows no temperature value likely has either no temperature sensor or a faulty temperature sensor installed. Existing Modules that did not originally have temperature sensors can be upgraded to include temperature sensors. Contact ANKOM for details.

Q23. Why are my modules with temperature sensors not communicating with the computer?

You must run GPM software version 9.7 or greater when operating modules with temperature sensors.

Q24. Can I save the graphs that are automatically generated on the GPM screen?

The Auto Graphing capability is meant to provide the user with a visual indication of the data being recorded during a gas study. However, the graphs themselves are NOT automatically stored after the study is complete.

To save the graphs from the Auto Graphing feature, you can either:

1) right click while on the graph screen and select "Save Image As . . .", or

2) right click while on the graph screen and select "Copy", or

3) do a Print Screen.

Please note that all of the raw data is stored in MS-Excel format.Therefore, you can always recreate a graph from the stored data by using the graphing feature within MS-Excel.


Q25. Why am I getting "error saving to xls file" messages now that I've set up my new GPM system?

This is most likely because the default "AutoSave location" is in a location that your computer may not have "write" access to. In addition, behind the scenes the program is also attempting to save a log file of every communication between the coordinator and each module to the same folder. These are both stored on C:\Program Files(x86)\ANKOMRF\Gas Pressure Monitor or C:\Program Files\ANKOMRF\Gas Pressure Monitor. If in C drive you see both folders, Program Files and Program Files (x86), the GPM program will save to the Program Files (x86) folder. Have your system administrator give your computer write access to the correct folder/file path and the error should be resolved.

Analytical/Results Troubleshooting

(Modules will have red caps)

Q1. How do I collect gas or liquid from the Gas Production System?

Q2. How do I convert pressure increase (psi) to volume of gas produced (ml)?

Q3. What is the volume of "dead space?"

Q4. Why do I see negative results at the beginning of my run?





Q1. How do I collect gas or liquid from the Gas Production System?

See Service Procedure 174.

Q2. How do I convert pressure increase (psi) to volume of gas produced (ml)?

See Service Procedure 175.

Q3. What is the volume of "dead space" between the vent valve and the septum on an RF module?


The volume of "dead space" between the vent valve and the septum on an RF module is .043 cubic inches for the BLACK capped modules, and .003 cubic inches for the RED capped modules.

Q4. Why do I see negative results at the beginning of my run?

If you purge the bottle with CO2 before the run, the CO2 in the head space will dissolve into the fluid until it becomes saturated. If CO2 absorption happens faster than the rate at which the gas is being produced, then the net result will be negative. To avoid this, you can do one of the following at the beginning of the run:
1) Purge the head space with CO2 and then let it sit under pressure to allow time for the CO2 to aborb into the liquid. Record pressure and determine saturation of the liquid when the pressure stops declining. Release the remaining pressure and immediately add the sample/substrate and start the experiment.
2) Bubble CO2 into the solution until it is completely saturated. Then fill the bottle head space with CO2 and start the experiment.