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Analytical Methods

Daisy Incubators

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Analytical FAQs

After soaking the F57 Filter Bags in acetone in preparation for an in vitro procedure, should I dry the bags in the oven? If so, for how long and at what temperature?

The F57 Filter Bags should not be oven dried after the acetone treatment.  Doing so can release the calendared set (heat and pressure used to flatten) we put on the filtration media from which the bags are made.  Simply allow the acetone to completely evaporate from the bags prior to filling the bags with sample.

What is a MoistureStop Desiccant Pouch, how is it used, and why should I use it?

The MoistureStop Desiccant Pouch is a small, airtight zipper bag, utilized for desiccating all of the Filter Bags that are used with ANKOM instrumentation. A single pouch can hold a full run of F57, F58 or XT4 bags at one time.  When folded, 6 IDF or SDF bags can also be placed into a MoistureStop Desiccant Pouch. Each time a bag has been removed from the desiccant pouch, the air can and should be pushed out. The "zipper" does not have to be used with every bag removal but some action should be taken to keep the air from entering the pouch while the just removed Filter Bag is being weighed. For example, air can be pushed out of the bag by laying it on a firm surface while compressing or flattening the air out of the pouch with your hand. 

Many instruments in the marketplace have the capacity to run 6 samples at a time.  After being dried, the beakers, flasks or crucibles are placed into a counter top or cabinet desiccator.  Once cooled, they are then removed one at a time and weighed. Each time the desiccator is opened to remove sample, moist ambient air is introduced inside the desiccator. However, because the lid or door of the desiccator is opened just 6 times, the samples that remain in the desiccator after each item is removed are affected very little by ambient moisture that may be introduced.

However, with Filter Bag Technology, generally larger numbers of Filter Bags are extracted at a time. As with the beakers, flasks and crucibles mentioned above, if the bags are placed in a desiccator after drying, each time the lid or door is opened to remove a bag, moist, ambient air is introduced. Because the desiccator is opened up to 24 times, the moisture can more readily affect the remaining bags. If a collapsible, ANKOM desiccant pouch is utilized, the air can be pushed out of the pouch each time a Filter Bag is removed. This will eliminate a build up of moisture on the remaining Filter Bags and allow for a more accurate and precise result. Every month it is possible to place the small desiccant packets into the oven at 100° - 105 ° C for a few hours to insure that the desiccant is renewed. In addition, to ensure the integrity of the zip lock bag, regular replacement should be considered.

Can the ANKOM Filter Bags be ashed?

The F57 filter bags are ash free and therefore can be used for ash determination. See D200 Service Procedure 7 for the Ashing Procedure.

Documents for This Question

Procedure Title Download
07 Ashing Procedure View PDF

How do I test for protein digestibility residue after Daisy incubation?

This analysis can be performed using the older Kjeldahl Method with filter bags. The automated combustion instruments (Dumas Method) can also be used. With some of the automated instrumentation, it may be difficult to get the entire filter bag into the "pellet" form necessary but many customers have been able to cut up the bag and make it fit.

Why am I getting Low Digestibility values?

Possible Causes:
1)  Sample size is too large.
2)  Bags were not acetone rinsed.
3)  The rubber baffle spacers fell off.

Possible Solutions:
1)  Ensure that the procedure is followed completely.
2)  Reduce sample size to .025 per procedure.
3)  Rinse bags with acetone and thoroughly dry prior to filling.
4)  Check baffle spacers.

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