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What is the run time for Vitamins and Cholesterol?
What is the run time for Vitamins and Cholesterol?
The ANKOMFLEX Vitamin and Cholesterol analysis run time is a little over two hours. Technician time is only 5-10 minutes of set-up prior to running the instrument. This includes weighing samples into each digestion vessel and loading the digestion vessels, recovery vessels and columns onto the instrument. After the method has finished, the user must reconstitute the isolate and quantify chromatographically.
What is the run time for Total Fat?
What is the run time for Total Fat?
The ANKOMFLEX Total Fat analysis run time is a little over two hours. Technician time is only 5-10 minutes of set-up prior to running the instrument. This includes weighing samples into each digestion vessel and loading the digestion vessels, recovery vessels and columns onto the instrument. After the method has finished, the user must transfer the isolated fat for gravimetric analysis or fatty acid profiling.
How many samples can you run in one day?
How many samples can you run in one day?
One ANKOMFLEX run includes four assays will be completed in approximately two hours. Therefore, in an 8-10 hour workday, the instrument can be run four times, resulting in 16 assays per day, and 80 assays per 5-day work week.
What is the max sample size you can process?
What is the max sample size you can process?
The ANKOMFLEX can process up to 10g of sample, depending on sample type.
Samples that typically require a large sample size, are samples with low analyte content, such as juice, or samples with poorly dispersed analyte, such as animal feeds.
If LOQ is an issue, please contact ANKOM for analytical support and guidance.
Samples that typically require a large sample size, are samples with low analyte content, such as juice, or samples with poorly dispersed analyte, such as animal feeds.
If LOQ is an issue, please contact ANKOM for analytical support and guidance.
What types of samples can be run?
What types of samples can be run?
All food and feed samples can be run on the ANKOMFLEX.
For vitamin methods, what are your saponification temperatures? Are they set or variable?
For vitamin methods, what are your saponification temperatures? Are they set or variable?
The ANKOMFLEX method saponifies at 75°C for 45min in accordance with official methods. However, the temperature can be reduced if desired and the duration of saponification can be adjusted.
Do you have issues with emulsions?
Do you have issues with emulsions?
The ANKOMFLEX employs SPE (Solid Phase Extraction) as the extraction method, therefore eliminating emulsions.
Can the ANKOM FLEX be used for extracting vitamin K?
Can the ANKOM FLEX be used for extracting vitamin K?
The expectation is vitamin K is an assay that can be run however the method has not been validated at this time. The ANKOMFLEX is a versatile instrument with many potential applications.
Can the ANKOM FLEX be used to extract cholesterol?
Can the ANKOM FLEX be used to extract cholesterol?
Yes, the extraction process, and method, are the same as the fat-soluble vitamin extraction.
Can I purchase a vitamin check sample?
Can I purchase a vitamin check sample?
Yes, a check sample can be purchased on the ANKOM website, SKU:FLEX65 https://www.ankom.com/product-catalog/check-sample-infant-formula
What material is used in the SPE column?
What material is used in the SPE column?
The SPE column contains silica-based sorbent.
Do we have to purchase the SPE columns and filters through ANKOM?
Do we have to purchase the SPE columns and filters through ANKOM?
The SPE columns and vessel filters can only be purchased through ANKOM.
Which official methods does the ANKOM FLEX analysis reference?
Which official methods does the ANKOM FLEX analysis reference?
The preprogrammed vitamin methods are based on a combination of the following official methods:
AOAC 2011.07: Determination of vitamin A in infant formula and adult nutritionals by UPLC-UV
EN-12823-1: Determination of vitamin A in foodstuffs by HPLC
EN-12822: Determination of vitamin E in foodstuffs by HPLC
AOAC 2011.07: Determination of vitamin A in infant formula and adult nutritionals by UPLC-UV
EN-12823-1: Determination of vitamin A in foodstuffs by HPLC
EN-12822: Determination of vitamin E in foodstuffs by HPLC
Does the FLEX have to be in a hood?
Does the FLEX have to be in a hood?
No. The instrument exhaust lines (both waste and vent lines) are trapped and ready to be connected to an exhaust system.
How do you dispose of the columns?
How do you dispose of the columns?
Sorbant Disposal Bags hold spent sorbant from four SPE columns while allowing solvents to evaporate overnight in a hood. Dispose of the empty SPE columns and Disposal Bags using your internal hazardous waste SOPs and SDS guidelines.
Sorbant Disposal Bags are available at: https://www.ankom.com/product-catalog/sorbent-disposal-bag-20-count
Sorbant Disposal Bags are available at: https://www.ankom.com/product-catalog/sorbent-disposal-bag-20-count