TDF Analyzer

To get this fault, one or more of the Paddle Temperature Sensors is reporting erroneous low data of  < 5^oC.  The fault message on the Touch Screen Display will indicate which station is having the problem and the heater to this station will be disabled.  If known, notate the temperature of the paddle when this fault occurred. The remaining stations will continue to run as normal. Depending on the time at which this fault occurred, one may want to disregard the test results from the suspect station.

There are three possible reasons for this fault; (1) faulty wiring from the paddle bar to the circuit board, (2) a faulty temperature sensor within the paddle bar, or (3) a faulty circuit board.

1. If the wiring from the paddle bar to the circuit board is faulty, the E1 fault will occur for all six positions. If this happens, there are a couple possibilities.  First turn power off to the instrument and open the electrical enclosure.  Contact ANKOM Technology for more detailed instructions. Inspect the white 3-pin connector labeled “J2” that plugs into the circuit board labeled “MUX-T”.  This connector plugs in near the top of the MUX-T circuit board. Inspect the bottom of the three black wires in the J2 connector and be sure it is securely plugged into the connector.  If it is loose or has become disconnected this will cause an E1 fault at all six positions. If the crimp terminal on this wire is loose or has become disconnected this will also cause this fault.  If this wire appears to be securely connected into the 3-pin connector, also check to see if there is continuity from the from the same wire in the J2 connector to the other end of this wire in the 25-pin connector that the paddle bar cable plugs into.  You will have to disconnect the 25-pin paddle bar connector that plugs at the bottom of the electrical enclosure to confirm this with a voltmeter. If this is identified as the problem the TDF15 Paddle Bar Wire Harness will need to be replaced. Contact ANKOM Technology for further assistance.
2. If the E1 fault is not related to all six positions then it is most likely related to the Temperature sensor within the paddle bar. When the run in progress is done, turn the instrument off and back on again.  Using the Diagnostics mode, select Temperatures and see if the station that reported the problem reading is still doing so.  If the problem reading is ongoing an E1 fault will display again.  If not, run a brief test.  Again using the Diagnostics mode, select Heater Test, press Paddle Heater Power Enable. Having done so, turn on all six paddle heaters. Monitor the temperatures closely to see if the E1 fault returns during heating of the paddles. Do not leave the instrument at this time as the heaters will stay on potentially overheating them if not controlled. Turn the heaters off when they reach 95˚C.  Allow the paddles to cool back to ambient temperature. If during the heating or cooling, the fault recurs, the Paddle Bar will need to be replaced. If the fault does not happen again, leave power on to the instrument overnight while in the Select a Function screen.  The program is always monitoring temperatures when power is on and if the fault occurs again during this time, the Paddle Bar will need to be replaced. If the fault does not recur during any of these tests, it is possible that a voltage fluctuation or static discharge near the sensor may have caused this fault.  The instrument may be put back into use. Keep record of any further E1 faults and if any do occur again, replace the Paddle Bar.
3. It is also possible that the MUX-T circuit board is faulty.  This is the least likely of the possible problems.  Contact ANKOM Technology if the other options have been worked through and the problem persists.

Refer to Service Procedure TS021 Paddle Bar Replacement for replacement of the Paddle Bar Assembly (TDF23).  This will require calibration of the temperature sensors and proper setting of the mixing stroke.  If the problem persists or any questions arise, contact ANKOM Technology for further assistance ([email protected] or 1-315-986-8090).

An E2 fault can be caused by cold buffer running through the instrument. If you store your buffer solutions in the refrigerator, bring them to room temperature before starting a run.

To get this fault, the thermistor to the In-line Heater is reporting an erroneous low reading of < 10˚C.

The fault message on the Touch Screen Display will indicate that the In-line Heater is disabled.  The In-line Heater operates during IDF water rinses and 95% ethanol pre-heat when filling the SDF bags before the precipitation phase.  The run in progress will continue in spite of this fault.  If known, note the temperature of the In-Line Heater when this fault occurred. Depending on a number of factors the SDF results from this run may not be accurate.  Some samples may be more sensitive to lower temperature alcohol during the precipitation phase than others.  If this fault occurred late in the 95% alcohol pre-heat / delivery, the alcohol may have nearly reached the desired temperature. It may be advisable to repeat the run after the problem is corrected. If a temperature of 8˚C is seen for the in-line heater, this is likely a false reading and would indicate a disconnect in the Thermistor circuit. Turn the power off and open the electrical enclosure. Identify the “MUX-T” circuit board.  Locate the 3-pin connector labeled “J10” and be sure that this is securely connected to its location on the MUX-T board and that the red and white wires do not pull out of the connector easily.  If the J10 connector is loose, connect it securely and see if this resolves the problem.  If the problem continues the TDF98 TDF Thermistor Assembly within the In-Line Heater will need to be replaced.  If the problem persists, there may be an issue with the MUX-T board.

For replacement of the TDF98 TDF Thermistor Assembly see Service Procedure TS018 In-line Heater Replacement and refer to the steps specific to replacing the thermistor.

For further assistance, contact ANKOM Technology at [email protected] or 1-315-986-8090.

To get this fault, the Temperature Sensor in one or more of the paddle heaters are reporting high temperatures. This could occur at different times under specific conditions.

• During the Amylase phase or when the instrument is idle if a temperature of over 105^°C is reported.
• During the cooling cycle (after the Amylase phase) if after 20 minutes the bags have failed to drop in temperature to 62°C.
• During the AMG or Protease phase if a temperature of over 70°C is reported.

Should any one of these conditions be met during a run, the run will be aborted. The E3 error message on the Touch Screen Display will indicate which station(s) are reporting the overtemp fault. This may be an actual overtemp condition or it may be erroneous due to a connectivity issue. Either way the heaters will be shut down by the program and the run is aborted. If this were to occur during an SDF precipitation, the heaters will be shut down, but the SDF precipitation will continue as normal.

To determine if the overtemp condition is real and the result of a heater that is failing to cycle or turn off, run the following test. Turn the power off to the instrument and allow the paddles to cool to room temperature. Now turn the power back on and from the Touch Screen Display, select Diagnostics. Press Temperatures to monitor the reported temperatures. At the same time, by touch, check the temperature of each paddle, especially the one that faulted earlier. If the suspect paddle begins getting warm, there is a problem with the paddle heater control. Turn the power off. This will require the replacement of the I/O-2 circuit board. Contact ANKOM Technology for further assistance ([email protected] or 1-315-986-8090).

If the previous test does not reveal a problem with the paddle(s) heating unregulated, take the following steps to identify the problem. Turn the TDF instrument power off and then back on.  From the Select a Function screen, press Diagnostics and select Temperatures. See if the suspect paddle heater is again reporting an abnormally high (false) reading while sitting at room temperature. If so, there is a fault in one of the temperature sensors and the Paddle Bar Assembly (TDF23) will need to be replaced. If the readings appear normal and the E3 fault does not recur, run a brief test. Again using the Diagnostics mode, select Heater Test, press Paddle Heater Power Enable. Having done so, turn on all six paddle heaters.  Monitor the temperatures closely to see if the E3 fault returns during heating of the paddles. Do not leave the instrument at this time as the heaters will stay on potentially overheating them if not controlled. Turn the heaters off when they reach 95°C.  Allow the paddles to cool back to ambient temperature. If during the heating or cooling, the fault recurs, the Paddle Bar will need to be replaced. If the fault does not happen again, leave power on to the instrument overnight while in the Select a Function screen.  The program is always monitoring temperatures when power is on and if the fault occurs again during this time, the Paddle Bar will need to be replaced. If the fault does not recur during any of these tests, it is possible that a voltage fluctuation or static discharge near the sensor may have caused this fault.  The instrument may be put back into use.  Keep record of any further E3 faults and if any do occur again, replace the Paddle Bar.

Refer to TDF Service Procedure #21 for replacement of the Paddle Bar Assembly (TDF23).  This will require calibration of the temperature sensors and proper setting of the mixing stroke.  If the problem persists or any questions arise, contact ANKOM Technology for further assistance ([email protected] or 1-315-986-8090.

This fault occurs when the Temperature Sensor in the In-Line Heater reports a temperature exceeding 135˚C.   The E4 error message on the Touch Screen Display will indicate the temperature at the time of the fault. When this fault occurs the instrument automatically aborts the current run.
 
The In-Line Heater operates during IDF water rinses and when filling the SDF bags with 95% alcohol.  Possible causes for the E4 fault include:
 
1.        Empty chemical container during In-line heater operation (causing an actual overheat)  
2.        A short in the thermistor wiring (causing a false high reading) or a malfunctioning
           MUX-T circuit board (causing a false high reading)  
3.        Malfunctioning Heater Relay or I/O-2 Circuit board (causing an actual overheat)  
For replacement parts and further assistance, contact ANKOM Technology at [email protected] or 1-315-986-8090.  For replacement of an in-line heater, click the In-Line Heater Replacement service procedure link shown below.

To get this fault, the temperature sensor in one or more of the paddle heaters is reporting low temperatures. This could occur at different times under specific conditions.

Should any one of these conditions be met during a run, the suspect heater/station will be shut down and stop heating.  The run will otherwise continue as normal. The E5 error message on the Touch Screen Display will indicate which station(s) are reporting the undertemp fault and what the temperature was at the time of the fault. This may be an actual undertemp condition or it may be erroneous due to a connectivity issue.  Either way the affected heater will be shut down by the program and the run will continue. If this were to occur during the IDF phase, with the heater being shut down, digestion will likely not occur as normal and the test results will not be reliable for this station.

Below is a list of potential causes of the E5 fault:

1. Bag breakage: Confirm that an IDF or Flow-thru bag did not break during one of the heating periods. If a bag were to leak near Clamp Bar B and if fluids were to get on one of the paddles, the liquid evaporation will drop the paddle temperature and possibly cause this fault.  If this has occurred, the instrument is sensing a real drop in temperature at the wetted paddle and no repair to the instrument is needed. Clean the area from the leaked bag. Test results from a broken bag should be disregarded.
2. Greater volume in the IDF or Flow-thru bags: Confirm that the volume calibration is accurate. Rerun the volume calibration as described in the operator's manual (QC/Calibration Checks section). If, for example, the volume calibration is significantly off, there could be an average of 50mls of buffer delivered instead of the required 40mls. This would create a delay in reaching temperature.
3. Pre-heat of paddles has been by-passed: In Diagnostics there is the option under Service Mode to "Bypass Amylase Phase Initial Heat-up." If this has been inadvertently pressed (stating "YES") this will bypass the paddle preheat to 50°C and take longer to heat up once the Buffer/Amylase is added. Make sure this option says "NO." This feature is typically used only for demo purposes.
4. Clamp Bar A is left open: If for any reason Clamp Bar A was left open during the Amylase phase heat up, there will be more evaporative cooling which will require more time ro heat the bags. NOTE: normally Clamp Bar A will close automatically after the final delivery to the IDF positions, but it can be re-opened.
5. Front cover left open or localized draft: Be sure to use the front safety cover, especially during the Amylase phase. If this cover is left open, and/or if there is a nearby A/C vent creating a draft toward the front of the instrument the Amylase phase heat-up will take longer. Also, if the TDF instrument is located inside a hood, turn off the air flow to the hood during the Amylase phase heat-up. The continued upward airflow will create a significant delay in reaching temperature. Addressing these may resolve the problem.
6. Alcohol - Buffer containers connected incorrectly: Ensure that the containers are connected correctly to the color-coded supply lines on the right side of the instrument. Confirm that alcohol did not inadvertently get put in the buffer container, and that buffer was not put in the alcohol containers. If either of these things happen, and alcohol gets dispensed to the IDF bags at the start of the Amylase phase, the alcohol will boil at a much lower temperature and not reach the target temperature of 97°C.

If the E5 fault was not the result of one of the above issues, run a brief test to see if all the paddle heaters are heating normally. Allow the paddles to return to room temperature. Obtain a stopwatch and 6 Flow-thru bags (used bags will work for this test). Use a heat sealer to seal the bottom edge of the bags. This will make removal later easier. Install these in the IDF positions. Connect the TDF70 Flush Tubing assembly to the HCL, 3 enzymes, buffer, water, and 2 alcohol connection points on either side of the instrument. Connect the middle connector to a filled Distilled Water container. From the Select a Function screen select a 991.43 TDF run. Answer all the prompts affirmative and press START. Press the "Display Temps/Pressures" button to monitor the temperature readings and watch the time that they reach 50°C. They should reach this temperature in about 2 to 2.5 minutes depending on the ambient starting temperature. Note the amount of time required. Reset your timer. As soon as Amylase and Buffer are delivered, mixing will start and restart your timer. Use the chart below to record every 2.5 minutes, the time it takes for each paddle to reach 90°C. T₀ is the end of the fluid delivery to the bags and start of mixing. Once they all reach 90°C, press ABORT to end the run and allow the bags to cool before removal.

Turn heaters off immediately after this test. If the suspect paddle heater takes significantly longer to heat than the others or fails to heat up, there is a problem with either the heater within the Paddle Bar Assembly or with the I/O2 circuit board. If the Paddles heat to the touch but one of the sensors shows an erroneous low reading, there is a problem with the sensor, and the Paddle Bar assembly will need to be replaced.  For further assistance, contact ANKOM Technology at [email protected] or 1-315-986-8090. Follow TDF Service Procedure #TS021 for replacement instructions of the Paddle Bar assembly (TDF23).
If one of the paddles fails to heat normally, and to rule out a problem with the I/O2 circuit board, remove the back and top panel of the Electrical Enclosure so that the I/O2 circuit board can be seen. Do not touch wiring. Individually turn on the paddle heaters one at a time checking to see that the appropriate LED light on the I/O2 circuit board lights up when the paddle is supposed to be heating. The following is a list of the Paddles and the corresponding LED #:

Turn heaters off after each test. If the appropriate LED is not lighting up, the problem may be with either the power supply or the I/O2 circuit board.  To test the power coming from the power supply to the individual paddle heater use a voltmeter set to DC. Connect the red lead on the voltmeter to the #20 position on the 24 volt DIN rail.  Now turn all the heaters on and test voltage as follows. On the back of the I/O2 circuit board are two 10-pin connectors labeled CN-3 and CN-4.  To test the power to each paddle, touch the black lead of the voltmeter to the following contact. 

You should detect 25.0 volts +/- 0.2 volts at each circuit.  If voltage is low there may a problem with the power supply or voltage adjustment.   For further assistance, contact ANKOM Technology at [email protected] or 1-315-986-8090. 

If all the LEDs are lighting up as normal and voltage is normal, the problem is with a paddle heater within the Paddle Bar Assembly.  For further assistance, contact ANKOM Technology at [email protected] or 1-315-986-8090.  Follow Service Procedure #TS021 for replacement instructions of the Paddle Bar assembly (TDF23).

This fault occurs when the Temperature Sensor in the In-Line Heater reports that the In-Line Heater is not reaching the required 110˚C within the 90 seconds allotted during an IDF water rinse.  The fault message on the Touch Screen Display will indicate that the In-line Heater is disabled.  The operator will be able to select OK and continue the run, but the cause should be investigated later.
 
The In-line Heater operates during IDF water rinses to facilitate rinsing of the IDF bag and to ensure that solutions when deposited into the SDF bag are nominally the same temperature as the pre-heated alcohol.  The run in progress will continue in spite of this fault.  If known, note the temperature of the In-Line Heater when this fault occurred.
 
Typically the test results from this run would not be affected by the cooler water rinse temperatures. Be sure that the sample in the IDF bag was rinsed down into the filter area of the bag and observe the precipitation phase for SDF.  If any anomalies are noted pay particular attention to the test results as it is possible results for certain sample types may be more sensitive to lower temperature during the precipitation phase than others.  It may be advisable to repeat the run after the problem is corrected. 
 
After the assay with the fault is complete, turn the power off to the instrument and turn it back on to clear the fault. Test the In-line heater under Diagnostics / Heater Test.  With a timer handy, observe how quickly the in-line heater heats up. Start the timer and immediately turn the In-line heater on and watch for it to get up to 70˚C.  It should reach 70˚C in 40-45 seconds. Turn it off as soon as 70˚C is reached. Confirm with an IR thermometer that the temperature reading on the touch screen display matches the actual temperature of the In-line heater based on the reading taken with the IR thermometer.  The two readings should be ± 5˚C of each other.   
 
If the In-line heater temperature appears to be frozen at 8˚C, there is likely a problem with a disconnected Thermistor wire at the MUX-T board or a lack of continuity in the Thermistor wiring.
 
If this fault is the result of a false low temperature reading, or if you need any further assistance, contact ANKOM Technology at [email protected] or 1-315-986-8090.
 
For replacement of an in-line heater, click the In-Line Heater Replacement service procedure link shown below.

This fault occurs when the Temperature Sensor to the In-Line Heater reports that the In-Line Heater is not reaching the required 80˚C within the 90 seconds allotted during an EtOH95 delivery.  The fault message on the Touch Screen Display will indicate that the In-line Heater is disabled.  The operator will be able to select OK and continue the run, but the cause should be investigated later.
 
The In-line Heater operates during the EtOH95 delivery to the SDF bags.  The run in progress will continue in spite of this fault.  If known, note the temperature of the In-Line Heater when this fault occurred. Typically the test results from this run would not be affected by the cooler ethanol temperatures. Observe the precipitation phase for SDF.  If any anomalies are noted pay particular attention to the test results as it is possible results for certain sample types may be more sensitive to lower temperature during the precipitation phase than others.  It may be advisable to repeat the run after the problem is corrected. 
 
After the assay with the fault is complete, turn the power off to the instrument and turn it back on to clear the fault. Test the In-line heater under Diagnostics / Heater Test.  With a timer handy, observe how quickly the in-line heater heats up. Start the timer and immediately turn the In-line heater on and watch for it to get up to 70˚C.  It should reach 70˚C in 40-45 seconds. Turn it off as soon as 70˚C is reached. Confirm with an IR thermometer that the temperature reading on the touch screen display matches the actual temperature of the In-line heater based on the reading taken with the IR thermometer.  The two readings should be ± 5˚C of each other.
 
If the In-line heater temperature appears to be frozen at 8˚C, there is likely a problem with a disconnected Thermistor wire at the MUX-T board or a lack of continuity in the Thermistor wiring.
 
If this fault is the result of a false low temperature reading, or if you need any further assistance, contact ANKOM Technology at [email protected] or 1-315-986-8090.
 
For replacement of an in-line heater, click the In-Line Heater Replacement service procedure link shown below.

This fault occurs when the Temperature Sensor reports a high temperature while the In-Line Heater is attempting to cool within the required timeframe.  Cooling is facilitated by flushing water through the lines of the In-line heater.  
 
The first time this occurs during a run, the operator will have the option to “CONTINUE” or “ABORT”.  If “CONTINUE” is selected the pump will resume flushing water through the in-line heater to bring the temperature down. If high temperature is reported again, the run will be aborted and an E9 In-line Heater Overtemp Fault will be displayed.  
 
Possible causes for the E9 fault include:
 
1.        Empty chemical container during In-line heater cool down cycle (causing a cool-down failure)  
2.        A short in the thermistor wiring or a malfunctioning MUX-T circuit board  
3.        Malfunctioning Heater Relay or I/O-2 Circuit board  
For replacement parts and further assistance, contact ANKOM Technology at [email protected] or 1-315-986-8090.  For replacement of an in-line heater, click the In-Line Heater Replacement service procedure link shown below.

An E10 fault is the result of one or more of the six pressure sensors reading a pressure above 20 psi during the initial buffer delivery at the beginning of the IDF phase or during the EtOH95 delivery to the SDF bags.  This fault will abort the run.  The fault screen will identify which line or lines are showing the high pressure.
 
The three main reasons for this fault are:
 
1.        An input valve (SDF or IDF) is failing to open, or pinch tubing is stuck closed.  
2.        There could be a blockage in one of the tubes or fill nozzles.  
3.        One of the pressure sensors could be faulty.  
For further assistance, contact ANKOM Technology at service @ankom.com or call 1-315-986-8090. 

An E11 fault is the result of the high pressure sensor reading input pressure below 35 psi.  N2 Input pressure is always being monitored and the fault could occur at any point during a run. The fault will interrupt the run and wait for the operator to correct the problem. This problem can result from one of the following causes:

An E12 fault is the result of the high pressure sensors reading input pressure above 100 psi.  N2 Input pressure is always being monitored and the fault could occur at any point during a run. The fault will interrupt the run and wait for the operator to correct the problem. This problem can result from one of the following causes:

For further assistance, contact ANKOM Technology at [email protected] or call 1-315-986-8090.

Refer to TDF Service Procedure #1, to diagnose a tubing pressure failure.

The Touch Screen Display will give you the option to Retry, Override, or Abort the run.  Retry will simply try the delivery again; Override will ignore the fault for the remainder of the run; and Abort will stop the run entirely.

The most likely cause of an E14 fault is an empty chemical container.  The fault screen will identify the container that was being drawn from when the error occurred.  The vacuum sensor is designed to monitor vacuum pressure on the supply lines when they are being drawn from.  When there is little or no vacuum on the line, this fault presents.  Refill the appropriate container as needed and select “Retry”.

Another potential cause of an E14 fault is a leak in any one of the supply tubes or fittings that would allow air to get pulled into a supply line. To test for this, under “Diagnostics”, run a “Motor Test and first “Set Valves”.  Open the supply line valve that generated the fault and open the Waste valve.  Set the volume to 40 mls and press “GO”.  Observe the supply lines at the rear of the instrument or tilt the instrument forward using the pivot mechanism to see the supply lines. Look for any air being drawn in at a hole in the tubing or at a crack in a fitting.  If this is observed, tubing or fittings may need to be replaced.  For further assistance contact ANKOM Technology at [email protected] or 1-315-986-8090.

Another possible cause of an E14 fault is an interruption in the Pump Motor Controller (PMC) operation.  If the PMC hesitates or stops because it has overheated or because the electronics on the circuit board have been compromised, the pause in operation will cause a drop in vacuum pressure and trigger an E14 fault.   This is possible even if the pause in operation were only for a second or two.  You should be able to retry and continue without a problem if that is the case; however, perform further testing after the run is complete.  After the run, perform a “Motor Test” under “Diagnostics” after first setting valves to Water Supply open and Waste open with all other valves closed. Set the volume to    250 mls – this will simulate the length in time of operation for a 95% alcohol delivery. Fill and plug in the water container. Press “GO” and listen for the Pump Motor operation.  Be attentive to any pause in the pump’s operation. The pump should run continuously for 6.5 – 8.5 minutes, depending on the volume calibration setting for your specific instrument. Stay with the instrument for the entire delivery.  Refill the water container when it nears empty, as it will draw more than 1 liter of water (250mls x 6).    If no pause in operation is noticed, allow the instrument to sit idle for 10 minutes and repeat the test.  If a hesitation or pause in operation is witnessed during the lengthy pump operation time the Pump Motor Controller will need to be replaced.  For further assistance contact ANKOM Technology at [email protected] or 1-315-986-8090.

The Touch Screen Display will give you the option to retry, override, or abort the run. 

• Retry will simply try the delivery again.
• Override will ignore the fault for the remainder of the run.
• Abort will stop the run entirely.

 
To analyze the problem, do the following:
When the instrument is not in the process of running an analysis, an E15 fault can be investigated by performing a Motor Test under Diagnostics.  From the Motor Test screen, press Set Valves and select the valve that caused the fault.  If this is not known, test each supply valve. Open only one supply valve at a time and close all others.  Open the Waste output valve and close the IDF and SDF output valves.  Be sure that there is adequate fluid in the reservoir that is being tested. Change the delivery volume to 20 ml and press GO.  Watch the vacuum reading on the screen.  Typical readings are between 0.7 – 1.5 psi.  A reading above 3 psi will generate an E15 fault (during a run).  If one of the supply lines shows a high reading, you have isolated the supply line that is causing the problem.
              
Possible causes of an E15 fault include:
1)  Clogged Filter
The most likely cause of an E15 fault at the Water, Buffer, EtOH78, EtOH95 or HCl lines is a clogged filter. Severely clogged filters may reduce delivery volumes and affect results. The filters are at the end of the draw tubes inside of the containers.  These should be checked and cleaned on a monthly basis and cleaned as needed. The filters can usually be cleaned with a soft bristle brush under running water. If they can't be cleaned, there are two spares of the plastic type container filters (used for water, alcohols and HCl/Acetic Acid) and one spare of the metal screen filters (used for buffer) included with the instrument on original purchase. Additional ones may be ordered as part 8202 (plastic filter) and part 8203 (metal screen filter).    
 
2)  Quick Disconnect Fitting not fully locked in place
A quick disconnect fitting functions as a valve that closes when disconnected or not fully connected. Quick disconnect fittings are found at the following reservoirs: enzymes, water, buffer, and alcohols.  Check to make sure that they cannot be pulled out without pressing the release button. If they can be pulled out without use of the release button they are not fully engaged and fluids will not be drawn. Push them into the adjoining fitting until you hear them snap in place. Confirm that they cannot be pulled out. It is also possible that the fittings may need to be replaced – see part 8193 (coupling body) and 8194 (coupling insert) on the ANKOM website.
 
3)  Worn tubing in the Enzyme Pinch Valve
An E15 fault at one of the Enzyme lines (Amylase, Protease, or AMG) could be due to worn tubing in the Enzyme Pinch Valve. As the tubing in the Enzyme Pinch Valve ages it may become stuck closed preventing the flow of enzymes. Review and follow Service Procedure #004, first replacing the tubing within the pinch valve.  These tubes should be replaced annually. Spare pinch valve tubing comes with the TDF instrument upon original purchase; additional ones can be ordered as part TDF71 Pinch Valve Tubing Set (21 pcs). Also inspect the supply lines to see if there is a clog or pinch in the line. These also may require replacement.  A set of all enzyme tubes can be ordered as part TDF67 Enzyme Tubing Replacement Kit.  If the problem persists, contact ANKOM Technology at [email protected] or 1-315-986-8090 for further assistance.
 
4)  Pinched or plugged supply line or a faulty supply valve
Other possible causes for an E15 fault are a pinched or plugged supply line or a faulty supply valve.  Inspect the supply line in question to see if such a problem can be seen visually.  After having cleaned and/or replaced the filter and having checked for fully engaged disconnect fittings, if the problem persists, contact ANKOM Technology at [email protected] or 1-315-986-8090 for further assistance.

The enzyme tubing may be clogged. Look at the enzyme tubing from the reservoirs to the Enzyme Pinch Valve and beyond to the Manifold. If there is any discoloration or gelled material in the tubing, replace the tubing.

Email ANKOM Technology at [email protected] or call 315-986-8090 to purchase your Enzyme Tubing Replacement Kit (TDF67).

Refer to TDF Service Procedure #4 for installation instructions.

Refer to TDF Service Procedure #4 and reference the steps related to relaxing or pre-stressing the replacement Silicone Tubing by rolling them with a rod or dowel.

This may occur if enzymes are used without a preservative. ANKOM recommends the use of a small concentration of Sodium Azide in the enzymes to prevent them from supporting bacterial, fungal or mold growth.Refer to the IDF/SDF Analysis section of the TDF Operator's Manual for more details.If enzyme tubes have become discolored, turned yellow or brownish, or if the enzyme has begun to gel in the tubes, they will require replacement.Refer to TDF Service Procedure #4 for instructions on doing this.Contact ANKOM Technology at [email protected] or call 315-986-8090 to purchase your Enzyme Tubing Replacement Kit (TDF67).

This may be the result of one of the IDF or SDF valves being stuck in a closed or partially closed position. Using the Diagnositcs screen select "Valve Test" and look for "IDF Inlet", "SDF Inlet" and "Waste" buttons. Run a brief test using the screen to open and close each valve. Confirm by looking at the pinch valve at the back of the instrument to see that it is opening and closing properly. If any of the valves are not opening properly, refer to TDF Service Procedure #8 to replace the pinch valve. The TDF29 Pinch Valve Assembly-IDF/SDF or TDF29.1 Pinch Valve Assembly- Waste can be ordered through the Product Catalog on this website or by calling 315-986-8090.

This problem could point to one of four possible issues with the instrument.

1) There could be a hole in that bag position’s fluid line after or within the PUMP causing a leak in the back of the instrument.  To determine of this is a problem, tilt the instrument forward and look for a leak from the silicone tubing. Open the PUMP doors and check for leaks from the Pump Tubes. Use Diagnostics to run a Pump Tube Test and check for leaks in the post-pump tubing specific to the problematic line.  Observe the pressure result from the line in question.  If there is a leak, a fitting or tube may need to be replaced. Contact ANKOM Technology at [email protected] or 1-315-986-8090 for replacement parts. If there are no leaks but the pressure result for the suspect line is low from the Pump Tube Test, this would point to a leak prior to the pump causing the pump to suck air through the hole OR a blockage in the line somewhere along the suspect tube length.

2) There could be a leak in the fluid line prior to the PUMP. To determine if there is a leak in the tubing line prior to the pump, run the following test. Under Diagnostics, run a Motor Test.  Set valves such that Waste is the only Output valve open and close all the Input valves.  Set the Motor Direction to REV (reverse). Put the ends of the Waste tubes in a beaker of water but before pulling them off of the tubing holder label them individually 1-6 for easy identification and re-attachment later. Leave the volume set to 10 mls and press GO.  Look for any leaks prior to the pump. Follow the suspect tube back from the PUMP to the MANIFOLD looking for any leaks along the suspect line.  If there is a leak, a fitting, tube, or MANIFOLD may need to be replaced.  Contact ANKOM Technology at [email protected] or 1-315-986-8090 for replacement parts. If there is no leak before or after the pump, proceed to issue #3 below.

3) There could be a blockage in a fluid line prior to the PUMP. To identify a blockage in a line prior to the PUMP, run the following test.  Under Diagnostics, run a Motor Test.  Set valves such that Waste is the only Output valve open and close all the Input valves.  Set the Motor Direction to REV (reverse). Put the ends of the Waste tubes in a beaker of water but before pulling them off of the tubing holder label them individually 1-6 for easy identification and re-attachment later. Disconnect the single (larger diameter tube) at the bottom of the MANIFOLD from the 6-way fitting. Leave the short (1 ½”) length of tube attached to the bottom of the MANIFOLD.  Leave the volume set to 10 mls and press GO.  Collect the fluid from the bottom of the MANIFOLD into a clean beaker.  Look for any particles, dried enzyme, or mold growth being washed out.  If contaminants are seen, repeat this but draw Ethanol in at the Waste lines and repeat until the collected fluid is clear.

4) There could be a blockage in a fluid line after the PUMP. To identify a blockage in a line after the pump, consider the following.  First it should be noted that by performing the procedure in #3 above, a blockage may have been flushed backward through the line and out through the MANIFOLD. In most cases performing the procedure in #3 will correct a blockage before or after the pump, but if it does not, contact ANKOM Technology at [email protected] or 1-315-986-8090 for additional diagnosing direction.
 
After identifying and resolving the problem, reconnect all tubing and repeat a Pump Tube Test to be sure each tube is being pressurized and holding pressure properly. Also run a Motor Test setting the Supply valve WATER to open and the IDF Output to open. Make sure the Motor Direction is FWD (forward) and place six beakers under the IDF delivery nozzles. Press GO and confirm fluid is delivered to each beaker equally.  Do the same with the SDF Output valve open and beakers under the SDF delivery nozzles.  If these tests pass, your instrument is ready to be put back into service.  

Some variability in filter times is normal.  Default filter time settings take into account much of this variability.  Default settings for IDF/SDF filtration are as follows: IDF after release of bar B (5.0 min), IDF after water rinses (1.0 min), IDF after EtOH rinses (1.0 min), SDF after release of bar D (10.0 min), SDF after EtOH rinses 3.0 min).  The following are several factors that may contribute to slow filtration (taking longer than the default settings).

Lower air pressure
Make sure that the low pressure regulator is set properly.  Our recommended setting for the low pressure regulator is 4.0 PSI (static).  Do not adjust the regulator during filtration.  If your regulator is set lower than this, adjust the regulator and run the instrument again.  It should also be noted that during filtration, time may be added if slow filtration is observed.  As filtration is occurring a button will appear on screen that states, "Filter Time +".  Each time this is pressed, 1 minute will be added to the filtration time.

Sample type
The IDF and SDF filter bags have a significantly greater filter surface area than is found in the Gooch filter-crucible. However, regardless of filter surface area, some sample types can be difficult to filter. Samples that become quite gelatinous in an aqueous solution (such as psyllium and chia seeds) can be difficult to filter.  To accommodate these types of samples in the ANKOM^TDF Dietary Fiber Analyzer we recommend the following options:

Add filter time before the assay begins
At the beginning of a run a series of user prompts appear on screen, one of which states, “Filter Minutes OK? (for an IDF/SDF run) or “SDF Filter minutes OK?” for a TDF run.  From this screen, you can add filter time if you are working with samples that require more filter time.  Familiarity with a particular sample may dictate 10 minutes of IDF filter time, for example. Be aware that any time changes will be stored to memory and will be there for the next run.  You will, however, be able to change this back to the default or another setting at the beginning of each run.

Add more filter time during the filtering process
During IDF or SDF filtration and during the water or alcohol rinses (when filtering is occurring) one can press the “Filter Time +” button to add one additional minute at a time, up to 50 additional minutes.  The user will need to be present at the time of filtering to take advantage of this option.

Use a smaller sample size
Reduce the sample size to 0.1 g to minimize the filter clogging effect of gelatinous sample types.

Use Diatomaceous Earth (DE) in the IDF bags 
Pre-weigh 1 g of DE for each IDF bag.  When starting the run, select YES for the screen that states, “Check pH manually?”. Select YES whether or not you plan to perform a manual pH check.  When the instrument pauses for the pH check (and after any pH check/adjustment is complete) add 1 g of DE to each bag.  Press the “Continue” button once DE is added. The DE will be mixed in during the AMG digestion.  Adding the DE at this point, rather than at the beginning of the run, minimizes the risk of bag wear during mixing by having abrasive DE in a hot bag.  Be sure that the DE in the IDF bags is factored into your blank values.  Use of this technique helps increase filter capacity.

Wrinkles in the Filter Bags at clamp bar A or C
There is always some air leakage from the filter bags during filtration at clamp bars A and C.  Larger wrinkles may exacerbate this to the point of needing to add time to the filtration.  If one filter bag is taking longer than the others to filter, time may be added using the "Filter Time +" button that will be available on screen during each filtration.  When setting up IDF and SDF filter bags try to keep them laying flat against the rear clamp bar before starting the run.

Clogged Spray Tip
If slow filtration is observed consistently at one particular station, inspect the spray tip at this station.  The white spray tip is friction fit onto a stainless steel tube.  Remove the spray tip at the IDF or SDF station and inspect under magnification. The TDF45 Illuminated Magnifier (10x) is recommended for this. There are six small orifices (holes) around the side of the spray tip.  Check to make sure they are clear and not clogged with debris.  This can be cleaned with a TDF94 0.020 inch or 0.5 mm drill bit and pressurized air.  You could also replace it with one of the extra spray tips that was sent with the instrument originally.  After the spray tip has been cleaned or replaced turn on nitrogen to the spray tips.  Using the "Diagnostics" screen, select "Valve Test" and then either "IDF N2" or "SDF N2" to turn the nitrogen on through the six spray tips.  Hold a beaker of water up to each of the six spray tips to make sure nitrogen is bubbling equally at all positions.  Spare spray tips can be ordered as part TDF44. 

Black rubber gasket on front clamp bar A or C may be dented
Inspect the black gasket seal area at the position in question on front clamp bar A or C.  See if there are any dents or low spots.  The best way to check this is to lay the gasket from the front of the clamp bars against a very flat (machined) surface and see if light shows through at the position in question.  If you can visually see a significant low spot or dent in the gasket at the position in question, this will contribute to a poor seal and slow filtration.  If you have a second TDF instrument, try switching the clamp bars.  Install filter bags at all six positions (either IDF or SDF) and close the upper and lower clamp bars keeping the bags as flat as possible to minimize leaks.  Using the "Diagnostics" screen, select "Valve Test" and either "IDF N2" or "SDF N2" to inflate the bags.  If the position in question now inflates its filter bag as quickly as the rest, the problem points to a dent or low spot on the original front clamp bar.  Contact ANKOM Technology at [email protected] or call 315-986-8090 for assistance.  A replacement Front Clamp Bar A-position can be ordered as part TDF73; a replacement Front Clamp Bar C-position can be ordered as part TDF75.

If sample is mixing poorly at one or a few positions, the mixing pads may not be making good contact with the bag and paddle behind it. Refer to TDF Service Procedure #10.

If sample is mixing poorly in general, an addition of flow control valves on the inrush and exhaust of nitrogen at the mixer piston will help.  These will be accessible from the rear of the instrument afford better mixing control.  Refer to TDF Service Procedure #30.  Also see link to part TDF113 Mixer Flow Control Valve Assembly.

Please click the "Tech Bulletin - TDF - Waste Tray Leak" link in the table below.

Refer to TDF Service Procedure #3 for instructions on changing the Pump Tubing.

The TDF68 Silicone Pump Tubing should be changed when the instrument prompts the user with an E13 warning for weak pump tubes. The pump tubes typically last 6 months under normal use.  Heavy usage may necessitate a more abbreviated time period between tubing changes.  

Email ANKOM Technology at [email protected] or call 315-986-8090 to purchase your Pump Tubing Replacement Kit (TDF68).

Refer to TDF Service Procedure #3 (TS003) for installation instructions.  

The enzyme tubing should be changed annually under normal use. Heavy use may necessitate a more abbreviated time period between tubing changes.

Email ANKOM Technology at [email protected] or call 315-986-8090 to purchase your Enzyme Tubing Replacement Kit (TDF67).

Refer to TDF Service Procedure #4 for installation instructions.

Following are two possible reasons why enzymes may not be drawn from the reservoir.

a. The silicone tubes may be worn or flattened from use. To inspect the tubing, refer to TDF Service Procedure TS005 Tube Replacement (Pinch Valves) and reference the steps related to performing a "Valve Test." Perform the "Valve Test" and follow the proceeding steps to open the Enzyme Pinch Bar and examine the tubing. The silicone tubes should be clear and fully opening on their own. If any of the silicone tubes are excessively flattened or stuck in a pinched or closed position on their own (without the pistons pushing against them), then the tubes need to be replaced with the spare 3 1/4" silicone tubing that was originally sent with the TDF instrument. A set of 21 replacement tubes (3 1/4") can be ordered as TDF71 Pinch Valve Tubing Set. It is recommended that all the pinch valve tubing be replaced at the same time to keep them on the same maintenance schedule (annually). This would include the IDF, SDF, and Waste pinch valves.

b. It is also possible that there is a blockage within the enzyme lines. Visually inspect the tubing from the enzyme reservoirs to the enzyme pinch valve to the manifold. If any crystals or dried enzymes are found, follow service procedure TS037 Enzyme Line Flush Procedure linked to below.  NOTE: It is recommended that when running methods AOAC2009.01, 2011.25 or 2017.16 that enzyme lines be flushed after every assay using this service procedure.  If necessary, replace the enzyme supply tubing – see service procedure TS004 Tubing Replacement (enzymes).

Refer to TDF Service Procedure # 14.

A typical TDF run requires approx. 1400L (50cu.ft.) nitrogen gas. ANKOM recommends nitrogen gas be used versus compressed air due to the fact that nitrogen is generally cleaner, drier, and reduces fire risk associated with volatile solvents. The nitrogen supply should be 6.9 bar (100 psi). During the run, the nitrogen is used to operate pressure cylinders and also to facilitate bag filtering. During the filtering stage, the nitrogen is regulated to .3 bar (4 psi) and flow rate is approx. 15L/min  (.5cu.ft.).

The TDF instrument requires the following bench space: 132 cm long (52") x 77cm deep (30") x 92 cm high (36"). 
For shipment, some parts are removed and the dimensions are: 107 cm long (42") x 51 cm deep (20") x 76 cm high (30").

The TDF instrument alone weighs about 65 Kgs (142 lbs).  With the controller, attachments and other spare parts the weight is about 71 kgs (157 Lbs). A rinse stand adds 2.75 kgs (6 lbs) to the shipping weight.  The total weight for shipping purposes, when the order includes a rinse stand, is 93.5 kgs (206 Lbs).

Yes. Please refer to TDF Service Procedure #19 for details on how to ship your TDF Analyzer to ANKOM Technology.

Maintenance procedures can be found under the section titled, "Periodic Maintenance" in your TDF Operator's Manual, and a maintenance checklist can be found in the TDF Maintenance Checklist.

You can access these documents using the links below.
 

If you do the de-fat procedure using ANKOM XT4 filter bags, after you complete the Kjeldahl digestion, there will be a white substance on top of the resulting liquid. This substance is the PTFE coating on the XT4 filter bags. This substance will NOT impact your results.