Which official methods does the ANKOM FLEX analysis reference?

The preprogrammed vitamin methods are based on a combination of the following official methods:

Vitamin & Cholesterol methods based on: AOAC 2011.07, EN-12823-1, EN-12822

Total Fat method: The test portion is treated under heated hydrochloric acid. The mixture is cooled and filtered. The residue is extracted from the filter with solvent. The extracted solvent is cleaned up with SPE technology, the solvent is evaporated, and fat is retained and measured gravimetrically.  

Crude Fat method: The test portion is submerged in heated solvent, prior to rinsing in cold solvent. After extraction, the solvent is evaporated, and fat is retained and measured gravimetrically.