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ANKOM FLEX Analyte Extractor

Part #

Automated Saponification to Isolation

The ANKOM FLEX Analyte Extractor's innovative design and automated technology supports multiple analysis configurations and preparation:  

  • Vitamin & Cholesterol methods based on: AOAC 2011.07, EN-12823-1, EN-12822
  • Total Fat method: The test portion is treated under heated hydrochloric acid. The mixture is cooled and filtered. The residue is extracted from the filter with solvent. The extracted solvent is cleaned up with SPE technology, the solvent is evaporated, and fat is retained and measured gravimetrically.  
  • Crude Fat method: The test portion is submerged in heated solvent, prior to rinsing in cold solvent. After extraction, the solvent is evaporated, and fat is retained and measured gravimetrically. 

For these analyses, the FLEX automatically digests, extracts, and evaporates multiple samples at one time, in about two to three hours, eliminating technician involvement. Isolated analytes can then be quantified gravimetrically (total fat, crude fat) or chromatographically (LC-DAD/FLD, LC-MS-MS, or GD-FID; Vitamin A, E, D, cholesterol, and fatty acid profiling).

  • Automates and simplifies fat-soluble vitamins and cholesterol, or crude and total fat analyses
  • Adjustable method parameters
  • Custom Methods Software available for purchase to create original methods  
  • Automatic chemical additions reduce chemical handling to improve safety
  • Eliminates bi-phase extractions & emulsions
  • Reduces technician labor by eliminating transfer steps
  • Reduces costs per assay  
  • Full record-keeping allows users to review, analyze, and troubleshoot historical data
  • Touchscreen interface is easy to use with step-by-step instructions  

Technical Specifications

Sample Types

Validated for all nine sections of the AOAC NIST Food-matrix Triangle 

Fat Ranges

0-100 %

# of Assays per run

4 assays in 2-2.5 hours, ~16 per day, 80 in a 5-day week  

Sample Size

Vitamins & Cholesterol: Up to 10 g
Fat: 1 g 

Operating Time

Total Fat: 2 hours 30 minutes
Crude Fat: 1 hour 20 minutes 
Vitamins & Cholesterol: 2 hours – 2 hours 15 minutes 

Instrument Dimensions

45.7 cm (18 in) L x 77.5 cm (30.5 in) W x 81.3 cm (32 in) H 


54.4 kg (120 lbs)

Power Requirements

100-120 V~ 50/60Hz 15A 
220-240 V~ 50/60Hz 8A

Nitrogen Requirements

N2 Supply 
Pressure: 5.5-6.9 bar (80-100 psi)
Flowrate: 10LPM at 6.9 bar minimum
Purity: Industrial Grade (Grade 4.8)- 99.998% purity or higher 

Fitting/adapter required to connect N2 supply to ¼” (6.35 mm) 
Polyurethane tubing (10 ft (3 m) length supplied by ANKOM)

Ambient Temperature Range

19-30 °C (66-86 °F)


20-60 % RH


Closed system with exhaust lines ready to connect to an exhaust system 


Four reservoirs programmable by the user for the required solutions 


Excess solutions are collected in the waste reservoir and can be discarded per internal SOPs

Consumable Type

SPE Columns and Digestion Filters