It is possible to follow a NDF with an ADF run using the same sample and Filter Bag. The only drawback is that some samples will produce a lower than expected ADF value when a NDF is run first. This is because certain components like pectin, tannins, glucans and fructans are removed by NDF solution but not by ADF solution. Therefore, these components are not present in the sample when the ADF follows the NDF. When you test these types of samples, you will have artificially lower ADF values than you would if you had only run an ADF without the NDF. This issue has nothing to do with the Filter Bags. They will hold up to both assays with no problem. In fact, you can even follow the ADF with a lignin procedure using the same bags if you wish.

Caution when drying the bags: When running a Lignin procedure or a sequential NDF/ADF or NDF/ADF/Lignin with the F57 Filter Bag, it is important not to dry the bags overnight after the NDF or ADF procedure. A drying timeframe of 2-4 hours at 100° to 105°C is sufficient to thoroughly dry the bags after each procedure. Extended drying times or too high of a temperature can compromise the bag's filtration media.