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High or Low Assay Results

Check to ensure the procedures sent with your instrument were followed. Some possible variables that may affect your results are:

General Problems:

  • ND chemicals can come out of solution. Heat (102 - 105°C +/- 2°C ) and stir to bring back into solution.
  • The temperature controller settings werer modified. This can produce improper heating.
  • Improper weighing technique. See weighing technique in the Operator's Manual.
  • Improper or non-use of the desiccant pouch. A standard desiccator will introduce moisture in the samples as it is being opened and closed. This will cause poor precision. The ANKOM Desiccant/Moisture Stop Assembly (X45) should be used. 

Low Values:

  • Grind is too fine.
  • Bag is not completely sealed.
  • Broken seam in bag is allowing sample to escape from bag.
  • Digestion run too long (ADF & Crude Fiber).

High Values:

  • The movement of the Bag Suspender is less than 1/2" (1.2 cm) up and down. Refer to your DELTA Operator's Manual section titled Periodic Maintenance. Follow steps to the check agitation system. 
  • Solution is not heated sufficiently. Perform ANKOM Boil Test (see DELTA Service Procedure 13).
  • For NDF, failure to use Alpha Amylase and/or Sodium Sulfite.
  • For ADF/NDF, failture to pre-treat samples with high fat content (>5%) with solvent.
  • Crude Fiber-always presoak with petroleum ether, not acetone.
  • Oven temp low, wet samples introduced next to dry samples-samples are not sufficiently dried.
  • Lack of or incorrect use of desiccant pouch. Check for holes in pouch. Desiccant pouch should be dried regularly to refresh desiccant. Follow directions on label. 
  • Rinse water not hot enough or not long enough. Water should be 70°C for NDF and ADF and 50°C for CF.  Rinse should run 5 minutes.
  • Digestion not run long enough.
  • Acetone re-used for removing water from bags post extraction.
  • Improper calculation of blank bag correction or using the wrong values in the formula.
  • Bag sealed too low.
  • Too much sample in the bag.
  • Sample not spread out in the bag.
  • Vessel lid was not closed tightly enough.
Related Documents: 

Boil Test

AttachmentSize
PDF icon AS013_Boil_Test.pdf391.79 KB

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