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The MoistureStop Desiccant Pouch is a small, airtight zipper bag, utilized for desiccating all of the Filter Bags that are used with ANKOM instrumentation. A single pouch can hold a full run of F57, F58 or XT4 bags at one time. When folded, 6 IDF or...
Refer to the Fat Extraction Process section of the ANKOM XT10 Extractor Operator's Manual. See subsection, Sample Preparation Procedure for Crude Fat.
Yes. For instructions on how to extract liquid samples, view ANKOM XT10 & XT15 Crude (Free) Fat Extraction of Liquid Samples and refer to the Sample Preparation section of the Operator’s Manual.
Yes, all samples should be treated the same during the drying process. The biggest and most common cause of artificially high crude fat results is incomplete drying in the pre-extraction phase. Because our technique uses the indirect method of...
Crude Fat results could be low for any of the following reasons: The extraction was not run long enough. The sample was not post-extraction dried enough. Fat migrated from a bag during the pre-dry step. Desiccant was either not used or incorrectly...
The XT instrument’s flowmeter should have a flow rate between 4-6 gallons per hour (If chilled coolant is used then the flow rate should be 2-3 gallons per hour). Note that this flow is intermittent and only turns on when cooling is required. When...
Petroleum ether is the most common solvent recommended for use in XT Extractors. Many other solvents will work depending on your objectives but may require upgrades to the method or instrument for them to run effectively. Hexane, for example, will...
This is likely due to a valve that may be "stuck" closed. If the instrument has not been used in a while, it is possible that the solution is simply to clean the valves and clean any debris or buildup that may have settled on the valve piston. To...
There is a leak. The most common cause is a leaky Q1 valve, which would cause cooling water to flow into the vessel during the heating stage. To test: Turn the instrument completely off but keep the water supply on. Check to see if water is running...
This can occur with samples that are very low in fat. Moisture left in low-fat samples after extraction is the issue. As AOCS Procedure AM 5-04 indicates, when the Filter Bags are removed from the Extractor, they should be placed in an oven at 100...
The XT10 and XT15 look very similar and have much in common. For example, both perform high temperature, high pressure soxhlet-like extractions on up to 15 samples at a time. The kinetics of the extraction provide an extraction time of no more than...
For XT10, see Service Procedure Fault ETS (X0S002). For XT15, see Service Procedure Fault ETS (X5S016).
Possible Causes: (1) The dial on the heat sealer may not be turned up high enough. To seal the XT4 Bag, the dial must be set at approximately "6." (2) The heating element is broken. On the sides of the Teflon cover are two shiny metal strips that...
To perform an extraction of oilseeds, refer to Oilseed Extractions (FOSFA Equivalent).
Research with high fat samples has given us the following understandings: Some samples with fats that have low melting point triacylglycerides or emulsifiers may encounter fat migration during hydrolysis. To improve fat retention, increase the level...
Some samples with large amounts of carbohydrates (starches and cellulose) may bind small quantities of solvent and require additional post drying. To remove the bound solvent, dry at 100-105° C for one to three hours.
Below is a list of steps to verify that the XT instrument is operating correctly. Always refer to your manual for complete, detailed information. Refer to XT15 Service Procedure Solvent, Vent, and Water Diagram (X5S006) for a four-page diagram that...
When using the Mojonnier mixture of solvents, a black residue may build up on the bottom of the vessel. If this coating becomes too thick, an "Overtemp Fault" "<Enter>" may occur. The black residue can be cleaned using a drill with a long-shafted...
There are three primary reasons for high Crude Fat results. (1) The bag is not completely sealed; therefore the sample gets lost during extraction. (2) A bag picks up surface fat from another sample. (3) Incomplete drying in the pre-extraction phase...
High Fat Samples are defined as any meat sample with a fat content >20% and any plant sample with a fat content >40%. See XT Service Procedure High Fat Sample Handling (XTS014).
For instruments with version 3.78 or newer software perform the following diagnostics routine: (1) Run a Line Charge two times with “All” selected with the flush tube assembly installed using deionized water. This will prime the lines in the...
An E11 fault is the result of the high-pressure sensor reading input pressure below 35 psi. N2 input pressure is always monitored and the fault could occur at any point during a run. The fault will interrupt the run and wait for the operator to...
The Silicone Pump Tubing ( Part #: TDF68 ) should be changed when the instrument prompts the user with an E13 warning for weak pump tubes. The pump tubes typically last 6 months under normal use. Heavy usage may necessitate a more abbreviated...
Yes. Please refer to TDF Service Procedure Return Shipping Instructions (TS019) for details.
Static electricity during the weighting process: The largest contributor to poor results is the effect of static electricity on the weighing process. Because plastic gloves can contribute to static electricity, only use anti-static gloves. To...
To properly prepare the IDF & SDF bags for Kjeldahl and Ashing procedures, refer to Section 17 of the TDF Operator’s Manual (Ash Determination) or view the following video: Kjeldahl & Ashing Bag Preparation .
Order the Fill Nozzle Replacement Kit ( Part #: TDF79 ) by contacting ANKOM Technology at (315) 986-8090 or by ordering online. Refer to the TDF Service Procedure Fill Nozzle Installation Procedure (TS014).
If you do the de-fat procedure using ANKOM XT4 filter bags, after you complete the Kjeldahl digestion, there will be a white substance on top of the resulting liquid. This substance is the PTFE coating on the XT4 filter bags. This substance will NOT...
This issue may occur if the instrument is not fully purged of the residual enzyme left in the supply lines, especially Trizma. ANKOM recommends flushing the enzyme supply lines with alcohol weekly when enzymes are being used. Further, we recommend...
The enzyme tubing may be clogged. Look at the enzyme tubing from the reservoirs to the Enzyme Pinch Valve and beyond to the manifold. If there is any discoloration or gelled material in the tubing, replace the tubing. Contact ANKOM Technology at...
Refer to TDF Service Procedure E13 Diagnosis (TS001) to diagnose tubing pressure failure.
The following are two possible reasons why enzymes may not be drawn from the reservoir. (1) The silicone tubes may be worn or flattened from use. To inspect the tubing, refer to TDF Service Procedure Tube Replacement (Pinch Valves) (TS005) and...
Do NOT run this instrument without an adequate DRY Nitrogen Supply. The instrument is powered by pneumatics that are not compatible with moisture, commonly found in compressed air. Nitrogen is also recommended as an extra precaution when handling...
This problem could point to one of four possible issues with the instrument. Investigate in the order below. (1) There could be a hole in the suspect bag position’s fluid line after or within the Pump causing a leak in the back of the instrument. To...
The touchscreen display will give you the option to Retry , Override , or Abort the run. Retry will simply try the delivery again. Override will ignore the fault for the remainder of the run. Abort will stop the run entirely. To analyze the problem...
We have found that it is best to slowly ramp up the digestion temperatures so that foaming in the digestion tubes is minimized. As a result, we would suggest the following times and temperatures: 15 minutes at 150°C, followed by 15 minutes at 250°C...
The enzyme tubing should be changed annually under normal use. Heavy use may necessitate a shorter timeframe between tubing changes. The Enzyme Tubing Replacement Kit ( Part #: TDF67 ) is available for purchase by contacting ANKOM Technology at (315)...
The TDF instrument requires the following bench space: 132 cm long (52") x 77cm deep (30") x 99 cm high (39"). For shipment, some parts are removed, and the dimensions are: 107 cm long (42") x 51 cm deep (20") x 76 cm high (30"). The TDF instrument...
To get this fault, the temperature sensor in one or more paddle heaters is reporting low temperatures. This could occur at different times under specific conditions such as: During paddle pre-heat, if any one of the paddles does not reach 50°C in...
Refer to the TDF Operator's Manual, sections "IDF/SDF Analysis" and "TDF Analysis."
Cause: This fault occurs when the temperature sensor in the in-line heater reports that the in-line heater is not reaching the required 110˚C within the 90 seconds allotted during an IDF water rinse. The fault message on the touchscreen display will...
An E2 Fault is declared when the thermistor to the in-line heater is reporting an erroneously low reading of < 10˚C. IMPORTANT: An E2 fault can be caused by cold buffer running through the instrument. If you store your buffer solutions in the...
Some sample types can be difficult to filter, even though the IDF and SDF filter bags have a significantly greater filter surface area than the Gooch crucible. Some samples become gelatinous in an aqueous solution (such as psyllium, chia seeds, or...
Protein determination is performed post the ANKOM TDF Dietary Fiber Analyzer. AOAC methods 991.43, 985.29, and 2001.03 suggest that the Kjeldahl method be used for determining protein content of the residue. AOAC methods 2009.01, 2011.25, 2017.16...
ANKOM recommends replacing the delivery tubing every one to two years depending on the use of the instrument. Order the Delivery Tube Replacement Set ( Part #: TDF126 ) by contacting ANKOM Technology at (315) 986-8090 or by ordering from the website...
Refer to TDF Service Procedure Tube Replacement Enzymes (TS004) and review the steps related to relaxing or pre-stressing the replacement silicone tubing by rolling them with a rod or dowel.
Some variability in filter times is normal. Default filter time settings encompass much of this variability. Default settings for IDF/SDF filtration are as follows: IDF after the release of bar B (five minutes), IDF after water rinses (one minute)...
If the sample is mixing poorly at one or a few positions, the mixing pads may not be making good contact with the bag and paddle behind it. Refer to TDF Service Procedure Mixing Pad Adjustment (TS010). If the sample is mixing poorly in general, the...
This fault indicates that one or more of the Paddle Temperature Sensors is reporting erroneously low data of < 5°C. The fault message on the Touch Screen Display will indicate which station is having the problem and the heater to this station will be...
Follow the steps in this instructional video to perform a pH adjustment: TDF Manually Measuring pH in the IDF Digestion . Or, reference section 13.22 of the TDF Operator’s Manual. Note: Measure within the appropriate pH range for your specific...