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The enzyme tubing should be changed annually under normal use. Heavy use may necessitate a shorter timeframe between tubing changes. The Enzyme Tubing Replacement Kit ( Part #: TDF67 ) is available for purchase by contacting ANKOM Technology at (315)...

This issue may occur if the instrument is not fully purged of the residual enzyme left in the supply lines, especially Trizma. ANKOM recommends flushing the enzyme supply lines with alcohol weekly when enzymes are being used. Further, we recommend...

The enzyme tubing may be clogged. Look at the enzyme tubing from the reservoirs to the Enzyme Pinch Valve and beyond to the manifold. If there is any discoloration or gelled material in the tubing, replace the tubing. Contact ANKOM Technology at...

Refer to TDF Service Procedure E13 Diagnosis (TS001) to diagnose tubing pressure failure.

The following are two possible reasons why enzymes may not be drawn from the reservoir. (1) The silicone tubes may be worn or flattened from use. To inspect the tubing, refer to TDF Service Procedure Tube Replacement (Pinch Valves) (TS005) and...

Do NOT run this instrument without an adequate DRY Nitrogen Supply. The instrument is powered by pneumatics that are not compatible with moisture, commonly found in compressed air. Nitrogen is also recommended as an extra precaution when handling...

The MoistureStop Desiccant Pouch is a small, airtight zipper bag, utilized for desiccating all of the Filter Bags that are used with ANKOM instrumentation. A single pouch can hold a full run of F57, F58 or XT4 bags at one time. When folded, 6 IDF or...

This problem could point to one of four possible issues with the instrument. Investigate in the order below. (1) There could be a hole in the suspect bag position’s fluid line after or within the Pump causing a leak in the back of the instrument. To...

The touchscreen display will give you the option to Retry , Override , or Abort the run. Retry will simply try the delivery again. Override will ignore the fault for the remainder of the run. Abort will stop the run entirely. To analyze the problem...

We have found that it is best to slowly ramp up the digestion temperatures so that foaming in the digestion tubes is minimized. As a result, we would suggest the following times and temperatures: : Preheat to 200°C; Clear at 300°Cfor 15min; Digest at...

An E11 fault is the result of the high-pressure sensor reading input pressure below 35 psi. N2 input pressure is always monitored and the fault could occur at any point during a run. The fault will interrupt the run and wait for the operator to...

The Silicone Pump Tubing ( Part #: TDF68 ) should be changed when the instrument prompts the user with an E13 warning for weak pump tubes. The pump tubes typically last 6 months under normal use. Heavy usage may necessitate a more abbreviated...

Yes. Please refer to TDF Service Procedure Return Shipping Instructions (TS019) for details.

Static electricity during the weighting process: The largest contributor to poor results is the effect of static electricity on the weighing process. Because plastic gloves can contribute to static electricity, only use anti-static gloves. To...

To properly prepare the IDF & SDF bags for Kjeldahl and Ashing procedures, refer to Section 17 of the TDF Operator’s Manual (Ash Determination) or view the following video: Kjeldahl & Ashing Bag Preparation .

Order the Fill Nozzle Replacement Kit ( Part #: TDF79 ) by contacting ANKOM Technology at (315) 986-8090 or by ordering online. Refer to the TDF Service Procedure Fill Nozzle Installation Procedure (TS014).

For instruments with version 3.78 or newer software perform the following diagnostics routine: (1) Run a Line Charge two times with “All” selected with the flush tube assembly installed using deionized water. This will prime the lines in the...

If you do the de-fat procedure using ANKOM XT4 filter bags, after you complete the Kjeldahl digestion, there will be a white substance on top of the resulting liquid. This substance is the PTFE coating on the XT4 filter bags. This substance will NOT...

ANKOM recommends replacing the delivery tubing every one to two years depending on the use of the instrument. Order the Delivery Tube Replacement Set ( Part #: TDF126 ) by contacting ANKOM Technology at (315) 986-8090 or by ordering from the website...

Protein determination is performed post the ANKOM TDF Dietary Fiber Analyzer. AOAC methods 991.43, 985.29, and 2001.03 suggest that the Kjeldahl method be used for determining protein content of the residue. AOAC methods 2009.01, 2011.25, 2017.16...

An E2 Fault is declared when the thermistor to the in-line heater is reporting an erroneously low reading of < 10˚C. IMPORTANT: An E2 fault can be caused by cold buffer running through the instrument. If you store your buffer solutions in the...

Some sample types can be difficult to filter, even though the IDF and SDF filter bags have a significantly greater filter surface area than the Gooch crucible. Some samples become gelatinous in an aqueous solution (such as psyllium, chia seeds, or...

The TDF instrument requires the following bench space: 132 cm long (52") x 77cm deep (30") x 99 cm high (39"). For shipment, some parts are removed, and the dimensions are: 107 cm long (42") x 51 cm deep (20") x 76 cm high (30"). The TDF instrument...

To get this fault, the temperature sensor in one or more paddle heaters is reporting low temperatures. This could occur at different times under specific conditions such as: During paddle pre-heat, if any one of the paddles does not reach 50°C in...

Refer to the TDF Operator's Manual, sections "IDF/SDF Analysis" and "TDF Analysis."

Cause: This fault occurs when the temperature sensor in the in-line heater reports that the in-line heater is not reaching the required 110˚C within the 90 seconds allotted during an IDF water rinse. The fault message on the touchscreen display will...

Cause: This fault occurs when the temperature sensor to the in-line heater reports that the in-line heater is not reaching the required 80˚C within the 90 seconds allotted during an EtOH95 delivery. The fault message on the touchscreen display will...

An E10 fault is the result of one or more of the six pressure sensors reading a pressure 20 psi above the baseline pressure during the buffer delivery to the IDF bags or during the EtOH 95 delivery to the SDF bags. This fault will abort the run. The...

AOAC 991.43 calls for filtration via a fritted crucible of 40-60 microns with Diatomaceous earth, or equivalent. Particle retention per the method is accomplished by adding diatomaceous earth contained in a fritted crucible. The fine filtration and...

Utilize a transfer pipette to handle this and follow the procedure below: (1) Weigh the pipette and tare the weight. (2) Fill the pipette with a liquid sample. (3) Place the pipette on the balance and record the weight. (4) Pipette out the liquid...

To correct this problem, try one or both of the following procedures: (1) During the de-fat procedure, do two 10-minute soaks in petroleum ether. This is particularly helpful with high fat (> than 20%) samples. (2) It is possible you have a sample...

In the past, we recommended customers to de-fat in XT4 bags. When the defatting process was completed, the XT4 bags were cut up and placed in the IDF bag. If the XT4 bag is cut into very small pieces, the sharp edges of the cut pieces can penetrate...

IMPORTANT: This message can be falsely declared if your instrument has been idle for a period of time. To reduce the chance of false declarations always run a line charge or two at the beginning of a routine, especially after a period of idle time (e...

Refer to TDF Service Procedure Tube Replacement Enzymes (TS004) and review the steps related to relaxing or pre-stressing the replacement silicone tubing by rolling them with a rod or dowel.

Some variability in filter times is normal. Default filter time settings encompass much of this variability. Default settings for IDF/SDF filtration are as follows: IDF after the release of bar B (five minutes), IDF after water rinses (one minute)...

If the sample is mixing poorly at one or a few positions, the mixing pads may not be making good contact with the bag and paddle behind it. Refer to TDF Service Procedure Mixing Pad Adjustment (TS010). If the sample is mixing poorly in general, the...

This fault indicates that one or more of the Paddle Temperature Sensors is reporting erroneously low data of < 5°C. The fault message on the Touch Screen Display will indicate which station is having the problem and the heater to this station will be...

Follow the steps in this instructional video to perform a pH adjustment: TDF Manually Measuring pH in the IDF Digestion . Or, reference section 13.22 of the TDF Operator’s Manual. Note: Measure within the appropriate pH range for your specific...

Refer to the TDF Operator's Manual, sections "IDF/SDF Analysis" and "TDF Analysis."

The touchscreen display will give you the option to Retry , Override , or Abort the run. Retry will simply try the delivery again. Override will ignore the fault for the remainder of the run. Abor t will stop the run entirely. Possible causes include...

To get this fault, the temperature sensor in one or more of the paddle heaters is reporting high temperatures. This could occur at different times under specific conditions such as: During the Amylase phase or when the instrument is idle if a...

Static electricity during the weighting process: The largest contributor to poor results is the effect of static electricity on the weighing process. Because plastic gloves can contribute to static electricity, only use anti-static gloves. To...

IDF filter bags have a shorter length of clear polypropylene material and a taller filter at the bottom of the bag. SDF filter bags have a longer length of clear polypropylene material and a shorter, wider filter at the bottom of the bag.

This fault occurs when the temperature sensor in the in-line heater reports a temperature exceeding 135˚C. The E4 error message on the touch screen display will indicate the temperature at the time of the fault. When this fault occurs the instrument...

This may be the result of one of the IDF or SDF valves being stuck in a closed or partially closed position. Using the Diagnostics screen select Valve Test and look for IDF Inlet, SDF Inlet , and Waste buttons. Run a brief test using the screen to...

Refer to TDF Service Procedure Long-Life Pump Tubing Replacement (TS028) for instructions.

Yes, there is a recommended procedure for the TDF Instrument for periods of non-use in Appendix C of the Operator's Manual for instructions. Following this procedure will help maintain clear fluid lines. The most current Operators Manual can be found...

This fault occurs when the temperature sensor reports a high temperature while the in-line heater is attempting to cool within the required timeframe. Cooling is facilitated by flushing water through the lines of the in-line heater. The first time...

Maintenance procedures can be found under the section titled, "Periodic Maintenance" in your TDF Operator's Manual and the TDF Preventative Maintenance Process Guide (TS047). A maintenance checklist can also be found below.

An E12 fault is the result of the high-pressure sensors reading input pressure above 100 psi. N2 Input pressure is always monitored, and the fault could occur at any point during a run. The fault will interrupt the run and wait for the operator to...