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The solenoid will become hot if the valve open check box is left checked. Normal valve openings are very brief and within a range of 10-1000 milliseconds, which does not cause the solenoid cylinder to get hot. If the Open Valves button is clicked or...

There may be a problem with the radio frequency (RF) chip attached to the circuit board. This is a dark green/blue colored chip with a black antenna. Refer to RF Service Procedure Chip and Board Replacement (RFS013) for help in identifying the part...

Please refer to Appendix E of the Operator's Manual.

Yes, Electrostatic Discharge (ESD) can cause the flash programming of the XBee chips in your #RF1, RF1X , # RF5 , and RF5X modules to become corrupted and stop communicating with the RF GPM software. Therefore, we highly recommend using an ANKOM Anti...

1. Verify that you have a Gen2 Coordinator: A Gen2 Coordinator will have a number that is less than 1,000. If you have connectivity issues and your coordinator number is greater than 1,000 you have a Gen3 system. In that case, contact ANKOM using the...

Use alcohol on both the underside of the modules and the bottles. Do NOT use an autoclave as the electronics and bottle coating will be damaged.

To update your GPM software: 1) Purchase the GPM software appropriate for your system. GEN2 systems (serial #’s <900) require GPM software version 11.4 ( Part #: RF3 ) GEN3 systems (serial #’s >1000) require GPM software version 15.0 ( Part #: RF3X )...

The valve will stay open for 90 seconds. The valve(s) can be closed before this time by clicking the Valve Open check box (to remove the check mark from the box), clicking the Close Valves button, or clicking the R ecord button. Since opening the...

When you press the record button on your GPM software screen, the program automatically stores your data in the file shown in the AutoSave location box. Therefore, if there has been a computer interruption during a study, you will be able to retrieve...

Refer to the Instrument Software Functionality section of the RF Gas Production System Operator's Manual.

Possible causes include: A loose or disconnected wire from the pressure sensor A faulty circuit board To diagnose, unplug the pressure sensor connector from the circuit board and plug in a pressure sensor connector from a working module into the same...

Yes, the ANKOM RF Gas Production System can monitor negative pressures. When performing oxygen consumption studies, a NaOH or strong base is used to scavenge the CO2 produced by the aerobic metabolism. This produces a negative pressure as the O2 is...

Due to the discontinuation of certain parts, ANKOM can no longer replace the RF programmed chip (Part #: RF45) and the Circuit Board (Part #: 7007) in Gen2 modules. All other repairs and replacement parts will continue to be available indefinitely.

The red septa can be used repeatedly during a study as it reseals when the needle is withdrawn. When pierced during gas sampling, it is important to use a small gauge needle and avoid puncturing the membrane in the same location. The red septa are...

Just prior to use, charge your rechargeable batteries (Part #: RF16 or RF46 ) for at least three hours using the 10-Station Battery Charger ( Part #: RF25 ). A fully charged battery when plugged into a module should read 6.7V or higher on the GPM...

No, the batteries (Part #: RF16 or RF46 ) should not get hot. If this happens, the battery pack may be defective, or the module's circuit board may have failed. Install another fully charged battery pack in the module. Then, perform the module...

You must run GPM software version 9.7 or greater when operating modules with temperature sensors.

The auto-graphing capability provides the user with a visual indication of the data being recorded during a gas study. However, the graphs themselves are NOT automatically stored after the study is complete. There are three ways to save the graphs...

Yes, the Dual Lead Rechargeable Battery ( Part #: RF46 ) allows you to connect the battery to both the module and the charger while the module is in use. Place the dual lead battery in the module and connect one lead to the module and the other to...

Yes, you can wash RF bottles in a laboratory dishwasher if the water temperature does NOT exceed 180°F (82°C) . Exposing the bottles to higher temperatures can damage the coating.

There are several possible reasons for a cumulative pressure drop or negative pressure readings. Refer to RF Service Procedure Curve Identification Chart (RFS010) for curve illustrations. Possible causes include: A leak in the system. First, be sure...

Refer to RF Service Procedure Module Testing (RFS001). First, check the pressure within the module. If the pressure is approaching 10 psi, stop using this module and unscrew the module top immediately to avoid over-pressurizing the bottle. If the...

The pressure sensors are accurate to +/-0.04 psi. Fluctuations in cumulative pressure beyond this correspond to either a leak or a fluctuation in temperature when a module is attached to a sealed bottle. If you check the "valve open" check box, leave...

Refer to RF Service Procedure Gas Sampling for Further Testing (RFS008).

Refer to RF Service Procedure CO2 Absorption (RFS009).

The MoistureStop Desiccant Pouch is a small, airtight zipper bag, utilized for desiccating all of the Filter Bags that are used with ANKOM instrumentation. A single pouch can hold a full run of F57, F58 or XT4 bags at one time. When folded, 6 IDF or...

Refer to XT15 Service Procedure Fault – ETS (X5S016). Note: Older XT15 software may present variations in fault wording. Should technical service be required, please photograph the fault screen so the technician can verify the software version.

This fault display means that at the end of the extraction, the level sensor detected solvent in the vessel (at the end of the run, the vessel should be empty and the sensor should not detect solvent). The instrument has either a Teflon Level Sensor...

When using the Mojonnier mixture of solvents, a black residue may build up on the bottom of the vessel. If this coating becomes too thick, an "Overtemp Fault" "<Enter>" may occur. The black residue can be cleaned using a drill with a long-shafted...

This can occur with samples that are very low in fat. Moisture left in low-fat samples after extraction is the issue. As AOCS Procedure AM 5-04 indicates, when the Filter Bags are removed from the Extractor, they should be placed in an oven at 100...

Petroleum ether is the most common solvent recommended for use in XT Extractors. Many other solvents will work depending on your objectives but may require upgrades to the method or instrument for them to run effectively. Hexane, for example, will...

Yes. For instructions on how to extract liquid samples, view ANKOM XT10 & XT15 Crude (Free) Fat Extraction of Liquid Samples and refer to the Sample Preparation section of the Operator’s Manual.

Possible Causes: The Q6 Valve (drain valve) is not opening correctly or there is a line blockage between the Q6 valve and the vessel. To best assist you, run a ~10 minute test. Please contact ANKOM with your results. To test: Verify that the Q6...

The XT instrument’s flowmeter should have a flow rate between 4-6 gallons per hour (If chilled coolant is used then the flow rate should be 2-3 gallons per hour). Note that this flow is intermittent and only turns on when cooling is required. When...

Crude Fat results could be low for any of the following reasons: The extraction was not run long enough. The sample was not post-extraction dried enough. Fat migrated from a bag during the pre-dry step. Desiccant was either not used or incorrectly...

Yes, all samples should be treated the same during the drying process. The biggest and most common cause of artificially high crude fat results is incomplete drying in the pre-extraction phase. Because our technique uses the indirect method of...

The XT10 and XT15 look very similar and have much in common. For example, both perform high temperature, high pressure soxhlet-like extractions on up to 15 samples at a time. The kinetics of the extraction provide an extraction time of no more than...

There is a leak. The most common cause is a leaky Q1 valve, which would cause cooling water to flow into the vessel during the heating stage. To test: Turn the instrument completely off but keep the water supply on. Check to see if water is running...

This fault display means the level sensor is indicating a full vessel when the vessel is actually empty. The level sensor has three parts: Glass Tip (visible inside the vessel) SICK Module (blue, found inside the electrical cabinet) Fiber Optic Cable...

There should not be any significant amount of solvent in the vent flask. If the solvent is dripping into the flask during the extraction, there may be a leak in valve Q7, and it may need to be cleaned or replaced. Refer to XT Service Procedure...

Possible Causes: (1) The dial on the heat sealer may not be turned up high enough. To seal the XT4 Bag, the dial must be set at approximately "6." (2) The heating element is broken. On the sides of the Teflon cover are two shiny metal strips that...

Refer to the Fat Extraction Process section of the XT15 Extractor Operator's Manual. Review the subsection titled Sample Preparation Procedure for Crude Fat.

For XT10, see Service Procedure Fault ETS (X0S002). For XT15, see Service Procedure Fault ETS (X5S016).

Refer to XT15 Service Procedure Solvent Drain & Flush (X5S019) linked below.

Refer to XT15 Service Procedure Changing the Solvent (X5S007).

There are three primary reasons for high Crude Fat results. (1) The bag is not completely sealed; therefore the sample gets lost during extraction. (2) A bag picks up surface fat from another sample. (3) Incomplete drying in the pre-extraction phase...

High Fat Samples are defined as any meat sample with a fat content >20% and any plant sample with a fat content >40%. See XT Service Procedure High Fat Sample Handling (XTS014).

Since your GPM software runs on a PC, power sources, the operating system (e.g., Windows) settings, as well as other programs and network connections on the PC can affect the GPM software. Power If your PC is being powered by a battery or an...

Yes. If you are looking to get some hands-on experience with the system before putting it to full use in a study, or if you observe results that you don't understand and want to verify that the ANKOM RF Gas Production System is functioning properly...

First, be sure Synthetic Grease w/PTFE ( Part #: RF41 ) was used on the lip of the bottle and the lip of the septa port on bottles so equipped. This aids in making a tight seal. Second, inspect the gasket which seals the module top to the bottle. It...