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Rinse water can come directly from the tap. It does not have to be distilled.

To perform an extraction of oilseeds, refer to Oilseed Extractions (FOSFA Equivalent).

Some samples with large amounts of carbohydrates (starches and cellulose) may bind small quantities of solvent and require additional post drying. To remove the bound solvent, dry at 100-105° C for one to three hours.

The FLEX Vitamin and Cholesterol analysis run time is a little over two hours. Technician time is only five to ten minutes of set-up prior to running the instrument. This includes weighing samples into each digestion vessel and loading the digestion...

The preprogrammed vitamin methods are based on a combination of the following official methods: Vitamin & Cholesterol methods based on: AOAC 2011.07, EN-12823-1, EN-12822 Total Fat method: The test portion is treated under heated hydrochloric acid...

To properly prepare the IDF & SDF bags for Kjeldahl and Ashing procedures, refer to Section 17 of the TDF Operator’s Manual (Ash Determination) or view the following video: Kjeldahl & Ashing Bag Preparation .

To determine how much DE to use with various sample types, refer to the Hydrolysis Procedure subsection titled Sample Categorization of the HCl Operator's Manual.

The MoistureStop Desiccant Pouch is a small, airtight zipper bag, utilized for desiccating all of the Filter Bags that are used with ANKOM instrumentation. A single pouch can hold a full run of F57, F58 or XT4 bags at one time. When folded, 6 IDF or...

When samples are dried for three hours after hydrolysis, they should be placed in a desiccant pouch ( Part #: X45 ) with a strip of pH paper. When the bags are fully dry, the pH paper will NOT show the presence of acid.

The F57 Filter Bags should not be oven-dried after the acetone treatment. Doing so can release the calendared set (heat and pressure used to flatten) we put on the filtration media from which the bags are made. Simply allow the acetone to completely...

During a crude fiber or acid detergent procedure, the time frame during which samples are exposed to 100°C solution(s) is extremely important. If the Fiber Analyzer begins a procedure at a temperature higher than 20°C, the instrument will heat the...

We have found that it is best to slowly ramp up the digestion temperatures so that foaming in the digestion tubes is minimized. As a result, we would suggest the following times and temperatures: 15 minutes at 150°C, followed by 15 minutes at 250°C...

The Blank Bag Correction allows for a weight adjustment based on actions that take place during solubilization. The resulting factor increases or decreases your sample bag weight. To determine the blank bag correction, include at least one empty...

When the neutral detergent solution gets cold, some of the components will begin to precipitate out of solution. As a result, the solution will turn milky white, and particles will be floating in it. These particles can be brought back into solution...

Petroleum ether is the most common solvent recommended for use in XT Extractors. Many other solvents will work depending on your objectives but may require upgrades to the method or instrument for them to run effectively. Hexane, for example, will...

Possible Causes: The rinse water supply temperature did not meet method standards. This may affect results with certain samples. Make sure the water supply has a temperature of no less than 50°C for Crude Fiber or no less than 70°C for ADF and NDF...

Yes. For instructions on how to extract liquid samples, view ANKOM XT10 & XT15 Crude (Free) Fat Extraction of Liquid Samples and refer to the Sample Preparation section of the Operator’s Manual.

Yes, all samples should be treated the same during the drying process. The biggest and most common cause of artificially high crude fat results is incomplete drying in the pre-extraction phase. Because our technique uses the indirect method of...

The ANKOM FLEX Total Fat analysis run time is just under two and a half hours. Technician time is only five to ten minutes of set-up prior to running the instrument. This includes weighing samples into each digestion vessel and loading the digestion...

The FLEX method saponifies at 75°C for forty-five minutes in accordance with official methods. However, the temperature can be reduced, if desired, and the duration of saponification can be adjusted.

No. The FLEX employs SPE (Solid Phase Extraction) as the extraction method, therefore eliminating emulsions.

While the expectation is that vitamin K is an assay that can be run on the FLEX, the method has not been validated at this time. The FLEX is a versatile instrument with many potential applications.

Yes. The extraction process, and method, are the same as the fat-soluble vitamin extraction.

Yes, all samples should be treated the same during the drying process. The biggest and most common cause of artificially high crude fat results is incomplete drying in the pre-extraction phase. Because our technique uses the indirect method of...

The ANKOM fiber instruments can analyze for either Acid Detergent Fiber (ADF), Neutral Detergent Fiber (NDF), or Crude Fiber. NDF consists of hemicellulose, cellulose, and lignin. ADF consists of cellulose and lignin. Acid Detergent Lignin (ADL)...

Protein determination is performed post the ANKOM TDF Dietary Fiber Analyzer. AOAC methods 991.43, 985.29, and 2001.03 suggest that the Kjeldahl method be used for determining protein content of the residue. AOAC methods 2009.01, 2011.25, 2017.16...

It is important that the rinse water going into the instrument is at least 50°C for Crude Fiber and 70°C for detergent fibers. For safety purposes, most hot water heaters are set lower than 70°C. In addition, if there is a substantial distance...

Refer to the TDF Operator's Manual, sections "IDF/SDF Analysis" and "TDF Analysis."

Test the rinse water with litmus paper during the last minute of the fourth hot water rinse. If the test indicates the presence of acid, repeat with fresh, hot water until a neutral pH is achieved.

Some sample types can be difficult to filter, even though the IDF and SDF filter bags have a significantly greater filter surface area than the Gooch crucible. Some samples become gelatinous in an aqueous solution (such as psyllium, chia seeds, or...

Neutral detergent consists of varying amounts of 4 dry chemicals and triethylene glycol. If you remove only a portion of the dry chemicals to make less than 20L of solution, it is impossible to know if the solution has the correct ratio of chemicals...

Amylase is utilized to help digest the starch in the sample. Even using expired Alpha Amylase, it is highly likely the non-starch components, as well as most of the starch, are gone. Put the Filter Bags in a 1 L beaker with 80 °C water and 8 ml of...

The XT10 and XT15 look very similar and have much in common. For example, both perform high temperature, high pressure soxhlet-like extractions on up to 15 samples at a time. The kinetics of the extraction provide an extraction time of no more than...

With the lignin procedure, two common errors will create false high values. (1) The first error is placing the F57 Filter Bags into the 72% sulfuric acid when there is moisture in the bags and samples. To avoid this situation, after the ADF procedure...

All food and feed samples can be run on the ANKOM FLEX.

Refer to the TDF Operator's Manual, sections "IDF/SDF Analysis" and "TDF Analysis."

AOAC 991.43 calls for filtration via a fritted crucible of 40-60 microns with Diatomaceous earth, or equivalent. Particle retention per the method is accomplished by adding diatomaceous earth contained in a fritted crucible. The fine filtration and...

In developing our hydrolysis process, we did extensive research into acid and base hydrolysis. We found that the acid hydrolysis did well with a variety of samples, including those normally hydrolyzed in a base solution, i.e., dairy products. As a...

Utilize a transfer pipette to handle this and follow the procedure below: (1) Weigh the pipette and tare the weight. (2) Fill the pipette with a liquid sample. (3) Place the pipette on the balance and record the weight. (4) Pipette out the liquid...

One ANKOM FLEX run includes four assays and will be completed in approximately two hours. Therefore, in an eight-to-ten-hour workday, the instrument can be run four times, resulting in sixteen assays per day, and eighty assays per five-day workweek...

The FLEX can process up to 10g of sample for vitamin and cholesterol analysis. The sample size maximum is dependent on analyte content, analyte homogeneity within the sample, and sample composition. Samples that typically require 10g sample sizes are...

Our grinding recommendation for samples to be extracted in our Fiber Analyzer is: 1 mm with a Wiley Mill 2 mm with a Cyclone Mill (such as UDY or Retsch)

This analysis can be performed using the older Kjeldahl Method with filter bags. The automated combustion instruments (Dumas Method) can also be used. With some of the automated instrumentation, it may be difficult to get the entire filter bag into...

Follow the steps in this instructional video to perform a pH adjustment: TDF Manually Measuring pH in the IDF Digestion . Or, reference section 13.22 of the TDF Operator’s Manual. Note: Measure within the appropriate pH range for your specific...

To understand how to mix DE with various sample types, view the How to mix DE with samples video.

It is possible to follow an NDF run with an ADF run using the same sample and Filter Bag. However, samples that are high in pectin, tannins, glucans, and fructans can produce lower-than-expected ADF values when NDF was run first. This is because...

High Fat Samples are defined as any meat sample with a fat content >20% and any plant sample with a fat content >40%. See XT Service Procedure High Fat Sample Handling (XTS014).

Refer to RF Service Procedure Gas Sampling for Further Testing (RFS008).