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If a Mojonnier solvent (pet ether, ethyl ether, ethanol) is utilized, a blank value of 0.0030g is normal. If pet ether or hexanes are used, a blank value of 0.0010g is normal. During the hydrolysis phase, fat migration may occur with samples that...

IDF filter bags have a shorter length of clear polypropylene material and a taller filter at the bottom of the bag. SDF filter bags have a longer length of clear polypropylene material and a shorter, wider filter at the bottom of the bag.

Use alcohol on both the underside of the modules and the bottles. Do NOT use an autoclave as the electronics and bottle coating will be damaged.

Bag breakage can occur when the heat sealer setting is not hot enough to ensure a good seal. Make sure each bag is sealed within 4mm of its open end. This section includes a narrow polypropylene strip that provides elasticity to the heat-sealed area...

Crude Fat results could be low for any of the following reasons: The extraction was not run long enough. The sample was not post-extraction dried enough. Fat migrated from a bag during the pre-dry step. Desiccant was either not used or incorrectly...

To update your GPM software: 1) Purchase the GPM software appropriate for your system. GEN2 systems (serial #’s <900) require GPM software version 11.4 ( Part #: RF3 ) GEN3 systems (serial #’s >1000) require GPM software version 15.0 ( Part #: RF3X )...

This fault occurs when the temperature sensor in the in-line heater reports a temperature exceeding 135˚C. The E4 error message on the touch screen display will indicate the temperature at the time of the fault. When this fault occurs the instrument...

This fault occurs when the temperature sensor reports a high temperature while the in-line heater is attempting to cool within the required timeframe. Cooling is facilitated by flushing water through the lines of the in-line heater. The first time...

Maintenance procedures can be found under the section titled, "Periodic Maintenance" in your TDF Operator's Manual and the TDF Preventative Maintenance Process Guide (TS047). A maintenance checklist can also be found below.

Possible cause: A power surge has occurred. Solution: Reprogram your controller using the controller menu setup in your Operator's Manual

Possible Causes: 1) Sample size is too large. 2) Bags were not acetone rinsed. 3) The rubber baffle spacers fell off. Possible Solutions: 1) Ensure that the procedure is followed completely. 2) Reduce sample size to .025 per procedure. 3) Rinse bags...

An E12 fault is the result of the high-pressure sensors reading input pressure above 100 psi. N2 Input pressure is always monitored, and the fault could occur at any point during a run. The fault will interrupt the run and wait for the operator to...

The TDF instrument produces about 475 ml per assay of filtrate waste consisting of about 75% ethanol-based solution, about 2,850 ml per run (6 stations). There is also water, buffer solution, and ethanol pumped through the system to flush lines and...

Over a period of years, the buffer valve, and possibly others, may begin to corrode the brass valve body. As of November 2022, the buffer valve in new instruments comes with a PEEK chemical-resistant valve body. However, older instruments may show...

1. Verify that you have a Gen2 Coordinator: A Gen2 Coordinator will have a number that is less than 1,000. If you have connectivity issues and your coordinator number is greater than 1,000 you have a Gen3 system. In that case, contact ANKOM using the...

The F57 and F58 Filter Bags are ash-free and therefore can be used for ash determination. Refer to the Ashing Procedure Service Procedure (AS034) linked below.

Refer to Service Procedure Insert Samples Into F58 Bags (AS032).

Possible Causes: The Q6 Valve (drain valve) is not opening correctly or there is a line blockage between the Q6 valve and the vessel. To best assist you, run a ~10 minute test. Please contact ANKOM with your results. To test: Verify that the Q6...

To minimize bag material abrasion, use the new bag suspender (as of March 2015 - Part #: F11 ). It has been redesigned to include rounded bottom points on each tray.

The XT instrument’s flowmeter should have a flow rate between 4-6 gallons per hour (If chilled coolant is used then the flow rate should be 2-3 gallons per hour). Note that this flow is intermittent and only turns on when cooling is required. When...

Yes, all samples should be treated the same during the drying process. The biggest and most common cause of artificially high crude fat results is incomplete drying in the pre-extraction phase. Because our technique uses the indirect method of...

The valve will stay open for 90 seconds. The valve(s) can be closed before this time by clicking the Valve Open check box (to remove the check mark from the box), clicking the Close Valves button, or clicking the R ecord button. Since opening the...

When you press the record button on your GPM software screen, the program automatically stores your data in the file shown in the AutoSave location box. Therefore, if there has been a computer interruption during a study, you will be able to retrieve...

Refer to the Instrument Software Functionality section of the RF Gas Production System Operator's Manual.

Possible causes include: A loose or disconnected wire from the pressure sensor A faulty circuit board To diagnose, unplug the pressure sensor connector from the circuit board and plug in a pressure sensor connector from a working module into the same...

The vessel temperature has exceeded the maximum limit. Possible Causes: The solution level is low in the vessel, but the level sensor is indicating full. The level sensor has two main components that work together. These components are a Level Sensor...

The instrument is experiencing a high-temperature fault. If your controller is set to 100°C, there are two possible reasons for this fault. (1) The agitation is not working properly during an assay. To check the agitation during an assay, remove the...

The ANKOM FLEX Total Fat analysis run time is just under two and a half hours. Technician time is only five to ten minutes of set-up prior to running the instrument. This includes weighing samples into each digestion vessel and loading the digestion...

The FLEX method saponifies at 75°C for forty-five minutes in accordance with official methods. However, the temperature can be reduced, if desired, and the duration of saponification can be adjusted.

No. The FLEX employs SPE (Solid Phase Extraction) as the extraction method, therefore eliminating emulsions.

Possible Causes: (1) The level sensor has failed to detect the liquid that is in the vessel. If the vessel is full and this fault screen appears, press <STOP> to drain the fluid. You may want to capture the solution and re-use it. Refer to DELTA...

While the expectation is that vitamin K is an assay that can be run on the FLEX, the method has not been validated at this time. The FLEX is a versatile instrument with many potential applications.

Yes. The extraction process, and method, are the same as the fat-soluble vitamin extraction.

This fault indicates a System Integrity Failure. One or more vessels did not reach or hold pressure properly. To resolve an MF1 fault: 1. Confirm the Low N2 setting is: 2.6 - 3.0 PSI. See Section 6.7.2 of the FLEX Operator’s Manual. 2. Tighten the...

The current software will not allow for remote monitoring. This functionality may be added in future software versions.

Yes. The flow path consists solely of chemically inert materials.

Possible Causes: If liquid has been manually poured into the vessel before the computer communicates with the appropriate valve to fill the vessel, this fault screen will appear. Open the vessel, if the vessel is full press "Y<ENTER>" to continue the...

Maintenance is low. At the end of the day, the digestion vessel spray nozzles, digestion vessel bases, and SPE column top rings must be wiped clean with a dampened towel. Some O-rings require replacement generally after about two hundred runs. Refer...

Below is a list of steps to verify that the XT instrument is operating correctly. Always refer to your manual for complete, detailed information. Refer to XT15 Service Procedure Solvent, Vent, and Water Diagram (X5S006) for a four-page diagram that...

When using the Mojonnier mixture of solvents, a black residue may build up on the bottom of the vessel. If this coating becomes too thick, an "Overtemp Fault" "<Enter>" may occur. The black residue can be cleaned using a drill with a long-shafted...

This analysis can be performed using the older Kjeldahl Method with filter bags. The automated combustion instruments (Dumas Method) can also be used. With some of the automated instrumentation, it may be difficult to get the entire filter bag into...

Refer to TDF Service Procedure Tube Replacement Enzymes (TS004) and review the steps related to relaxing or pre-stressing the replacement silicone tubing by rolling them with a rod or dowel.

Some variability in filter times is normal. Default filter time settings encompass much of this variability. Default settings for IDF/SDF filtration are as follows: IDF after the release of bar B (five minutes), IDF after water rinses (one minute)...

If the sample is mixing poorly at one or a few positions, the mixing pads may not be making good contact with the bag and paddle behind it. Refer to TDF Service Procedure Mixing Pad Adjustment (TS010). If the sample is mixing poorly in general, the...

This fault indicates that one or more of the Paddle Temperature Sensors is reporting erroneously low data of < 5°C. The fault message on the Touch Screen Display will indicate which station is having the problem and the heater to this station will be...

Follow the steps in this instructional video to perform a pH adjustment: TDF Manually Measuring pH in the IDF Digestion . Or, reference section 13.22 of the TDF Operator’s Manual. Note: Measure within the appropriate pH range for your specific...

The Overtemp message happens when the ANKOM HCl Instrument is heating but there is no solution in the vessel. The quartz heaters will heat the vessel and the ETS (Emergency Temperature Switches) will trip. Possible Causes: The instrument was not...

View the HCL Periodic Maintenance video .

To understand how to mix DE with various sample types, view the How to mix DE with samples video.

Refer to the TDF Operator's Manual, sections "IDF/SDF Analysis" and "TDF Analysis."