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The enzyme tubing should be changed annually under normal use. Heavy use may necessitate a shorter timeframe between tubing changes. The Enzyme Tubing Replacement Kit ( Part #: TDF67 ) is available for purchase by contacting ANKOM Technology at (315)...

This issue may occur if the instrument is not fully purged of the residual enzyme left in the supply lines, especially Trizma. ANKOM recommends flushing the enzyme supply lines with alcohol weekly when enzymes are being used. Further, we recommend...

The enzyme tubing may be clogged. Look at the enzyme tubing from the reservoirs to the Enzyme Pinch Valve and beyond to the manifold. If there is any discoloration or gelled material in the tubing, replace the tubing. Contact ANKOM Technology at...

Refer to TDF Service Procedure E13 Diagnosis (TS001) to diagnose tubing pressure failure.

The following are two possible reasons why enzymes may not be drawn from the reservoir. (1) The silicone tubes may be worn or flattened from use. To inspect the tubing, refer to TDF Service Procedure Tube Replacement (Pinch Valves) (TS005) and...

Do NOT run this instrument without an adequate DRY Nitrogen Supply. The instrument is powered by pneumatics that are not compatible with moisture, commonly found in compressed air. Nitrogen is also recommended as an extra precaution when handling...

The MoistureStop Desiccant Pouch is a small, airtight zipper bag, utilized for desiccating all of the Filter Bags that are used with ANKOM instrumentation. A single pouch can hold a full run of F57, F58 or XT4 bags at one time. When folded, 6 IDF or...

Replacement of the dryer completely depends upon how often it is used to dry hydrolysis samples. The filter should be changed when the filter bags do not dry sufficiently after hydrolysis at 110°C for three hours (adding pH paper in the desiccant...

When using the ANKOM Rapid Dryer to dry acid hydrolysis samples, the airflow through the special hydrolysis filter ( Part #: RD2 ) will eventually become too limited and the filter will need to be changed. To check the air filter for proper airflow...

When samples are dried for three hours after hydrolysis, they should be placed in a desiccant pouch ( Part #: X45 ) with a strip of pH paper. When the bags are fully dry, the pH paper will NOT show the presence of acid.

The F57 Filter Bags should not be oven-dried after the acetone treatment. Doing so can release the calendared set (heat and pressure used to flatten) we put on the filtration media from which the bags are made. Simply allow the acetone to completely...

This problem could point to one of four possible issues with the instrument. Investigate in the order below. (1) There could be a hole in the suspect bag position’s fluid line after or within the Pump causing a leak in the back of the instrument. To...

The touchscreen display will give you the option to Retry , Override , or Abort the run. Retry will simply try the delivery again. Override will ignore the fault for the remainder of the run. Abort will stop the run entirely. To analyze the problem...

During a crude fiber or acid detergent procedure, the time frame during which samples are exposed to 100°C solution(s) is extremely important. If the Fiber Analyzer begins a procedure at a temperature higher than 20°C, the instrument will heat the...

We have found that it is best to slowly ramp up the digestion temperatures so that foaming in the digestion tubes is minimized. As a result, we would suggest the following times and temperatures: : Preheat to 200°C; Clear at 300°Cfor 15min; Digest at...

Yes, Electrostatic Discharge (ESD) can cause the flash programming of the XBee chips in your #RF1, RF1X , # RF5 , and RF5X modules to become corrupted and stop communicating with the RF GPM software. Therefore, we highly recommend using an ANKOM Anti...

To properly set up your instrument for the various fiber analyses, refer to the A2000 Operator's Manual.

The extraction process utilizes positive pressure at ~2.8psi.

We suggest a purity of 99.998% nitrogen or purer as it is used to expedite solvent evaporation. We have not tested less pure nitrogen and therefore cannot validate that it will not adversely affect analyte recovery.

No. The instrument exhaust lines (both waste and vent lines) are trapped and ready to be connected to an exhaust system.

A "drain error" usually occurs if the level sensor senses the solution in the vessel after the drain has opened. If the vessel is empty, and the level sensor indicates "Full," the level sensor must be fixed. If the vessel is full of solution after...

Possible Causes: The controller needs to be reset. See DELTA Service Procedure Controller Temp Menu Setup (DES015) to determine if menu settings are incorrect and to make the appropriate changes. If the controller reset doesn't resolve the problem...

Possible Causes: The power cord is not plugged into the unit or power source. The main power switch is not turned on. One of the system circuit breakers (fuses) needs to be replaced. Refer to DELTA Service Procedure Fuse Replacement (DES025).

Refer to Service Procedure High Blank Bag Values (DES035).

The Blank Bag Correction allows for a weight adjustment based on actions that take place during solubilization. The resulting factor increases or decreases your sample bag weight. To determine the blank bag correction, include at least one empty...

When the neutral detergent solution gets cold, some of the components will begin to precipitate out of solution. As a result, the solution will turn milky white, and particles will be floating in it. These particles can be brought back into solution...

Depending on what you are testing, refer to section ADF Analysis, NDF Analysis, or Crude Fiber Analysis in the DELTA, A200, or A2000 Fiber Analyzer Operator's Manual.

Refer to XT15 Service Procedure Fault – ETS (X5S016) or Refer to XT10 Service Procedure Fault - ETS (X0S002). Note: Older XT10 or XT15 software may present variations in fault wording. Should technical service be required, please photograph the fault...

This fault display means that at the end of the extraction, the level sensor detected solvent in the vessel (at the end of the run, the vessel should be empty and the sensor should not detect solvent). The instrument has either a Teflon Level Sensor...

When using the Mojonnier mixture of solvents, a black residue may build up on the bottom of the vessel. If this coating becomes too thick, an "Overtemp Fault" "<Enter>" may occur. The black residue can be cleaned using a drill with a long-shafted...

This can occur with samples that are very low in fat. Moisture left in low-fat samples after extraction is the issue. As AOCS Procedure AM 5-04 indicates, when the Filter Bags are removed from the Extractor, they should be placed in an oven at 100...

Petroleum ether is the most common solvent recommended for use in XT Extractors. Many other solvents will work depending on your objectives but may require upgrades to the method or instrument for them to run effectively. Hexane, for example, will...

A "Low Pressure" error occurs when the vessel does not have at least 3 psi after 13 minutes. Possible Causes: (1) The lid was not tight. You do not have to over tighten the lid, but it needs to be snug. Usually, a finger tight + 1/4 turn is adequate...

The solenoid will become hot if the valve open check box is left checked. Normal valve openings are very brief and within a range of 10-1000 milliseconds, which does not cause the solenoid cylinder to get hot. If the Open Valves button is clicked or...

There may be a problem with the radio frequency (RF) chip attached to the circuit board. This is a dark green/blue colored chip with a black antenna. Refer to RF Service Procedure Chip and Board Replacement (RFS013) for help in identifying the part...

Please refer to Appendix E of the Operator's Manual.

For illustrations of melted trays, see A200 Service Procedure Temp Probe Replacement (FS016). Bag suspender trays can melt when there is not enough solution in the vessel to cover all of the trays while the instrument's heat is activated. Possible...

This is likely due to a valve that may be "stuck" closed. If the instrument has not been used in a while, it is possible that the solution is simply to clean the valves and clean any debris or buildup that may have settled on the valve piston. To...

There is a leak. The most common cause is a leaky Q1 valve, which would cause cooling water to flow into the vessel during the heating stage. To test: Turn the instrument completely off but keep the water supply on. Check to see if water is running...

Possible Causes: When the start button is pushed, the computer tells the appropriate valve to open and allow flow from the cubitainer into the vessel. If the liquid is manually poured into the vessel before the computer communicates with the...

The TDF instrument requires the following bench space: 132 cm long (52") x 77cm deep (30") x 99 cm high (39"). For shipment, some parts are removed, and the dimensions are: 107 cm long (42") x 51 cm deep (20") x 76 cm high (30"). The TDF instrument...

To get this fault, the temperature sensor in one or more paddle heaters is reporting low temperatures. This could occur at different times under specific conditions such as: During paddle pre-heat, if any one of the paddles does not reach 50°C in...

This can occur with samples that are very low in fat. Moisture left in low-fat samples after extraction is the issue. As AOCS Procedure AM 5-04 indicates, when the Filter Bags are removed from the Extractor, they should be placed in an oven at 100...

Possible Causes: (1) The vent tube or drain tube is blocked. The blockage is caused by water trapped in the line. It is very important that the vent and drain tubes run downhill and are not submerged in water. If the vent tube is blocked, a vacuum is...

Possible Causes: (1) The controller needs to be reset. Refer to Service Procedure Controller Temp Menu Setup (AS015) to determine if menu settings are incorrect and, if so, make the appropriate changes. (2) The RTD may need to be replaced. Contact...

Possible Causes: The power cord is not plugged into the unit or power source. The main power switch is not turned on. One of the system circuit breakers (fuses) needs to be replaced. Refer to Service Procedure Fuse Replacement (AS025).

Refer to the TDF Operator's Manual, sections "IDF/SDF Analysis" and "TDF Analysis."

Yes, the ANKOM RF Gas Production System can monitor negative pressures. When performing oxygen consumption studies, a NaOH or strong base is used to scavenge the CO2 produced by the aerobic metabolism. This produces a negative pressure as the O2 is...

Cause: This fault occurs when the temperature sensor in the in-line heater reports that the in-line heater is not reaching the required 110˚C within the 90 seconds allotted during an IDF water rinse. The fault message on the touchscreen display will...

An E2 Fault is declared when the thermistor to the in-line heater is reporting an erroneously low reading of < 10˚C. IMPORTANT: An E2 fault can be caused by cold buffer running through the instrument. If you store your buffer solutions in the...