TDF Analyzer

This fault indicates that one or more of the Paddle Temperature Sensors is reporting erroneously low data of  < 5^oC.  The fault message on the Touch Screen Display will indicate which station is having the problem and the heater to this station will be disabled.  Make a note of the temperature of the paddle when this fault occurred, if possible. The remaining stations will continue to run normally. Depending on the time at which this fault occurred, one may want to disregard the test results from the suspect station.

There are three possible reasons for this fault; (1) faulty wiring from the paddle bar to the circuit board, (2) a faulty temperature sensor within the paddle bar, or (3) a faulty circuit board.

1. If the E1 fault is reported for all six positions, the wiring from the paddle bar to the circuit board may be faulty.  To troubleshoot, turn power off to the instrument and open the electrical enclosure.  Inspect the white 3-pin connector labeled “J2” that plugs into the circuit board labeled “MUX-T”.  This connector plugs in near the top of the MUX-T circuit board. Inspect the bottom of the three black wires in the J2 connector and be sure it is securely plugged into the connector.  If it is loose or has become disconnected this will cause an E1 fault at all six positions. If the crimp terminal on this wire is loose or has become disconnected this will also cause this fault.  If this wire appears to be securely connected into the 3-pin connector, also check to see if there is continuity from the from the same wire in the J2 connector to the other end of this wire in the 25-pin connector that the paddle bar cable plugs into.  You will have to disconnect the 25-pin paddle bar connector that plugs at the bottom of the electrical enclosure to confirm this with a voltmeter. If this is identified as the problem, the TDF15 Paddle Bar Wire Harness will need to be replaced. Contact ANKOM Technology for further assistance.
2. If the E1 fault is not related to all six positions then it is most likely related to the Temperature sensor within the paddle bar. When the routine in progress is done, turn the instrument off and back on again.  Using the Diagnostics mode, select Temperatures and see if the station that reported the problem reading is still doing so.  If the problem reading is ongoing an E1 fault will display again.  If not, run a brief test.  Again using the Diagnostics mode, select Heater Test, press Paddle Heater Power Enable. Having done so, turn on all six paddle heaters. Monitor the temperatures closely to see if the E1 fault returns during heating of the paddles. Do not leave the instrument at this time as the heaters will stay on potentially overheating them if not controlled. Turn the heaters off when they reach 95˚C.  Allow the paddles to cool back to ambient temperature. If during the heating or cooling, the fault recurs, the Paddle Bar will need to be replaced. If the fault does not happen again, leave power on to the instrument overnight while in the Select a Function screen.  The program is always monitoring temperatures when power is on and if the fault occurs again during this time, the Paddle Bar will need to be replaced. If the fault does not recur during any of these tests, it is possible that a voltage fluctuation or static discharge near the sensor may have caused this fault.  The instrument may be put back into use. Keep record of any further E1 faults and if any do occur again, replace the Paddle Bar.
3. It is also possible that the MUX-T circuit board is faulty.  This is the least likely of the possible problems.  Contact ANKOM Technology if the other options have been attempted and the problem persists.

 

Refer to Service Procedure TS021 Paddle Bar Replacement for replacement of the Paddle Bar Assembly (TDF23).  This will require calibration of the temperature sensors and proper setting of the mixing stroke.  If a new paddle or paddle bar assembly is needed, contact ANKOM Technology prior to ordering at https://www.ankom.com/contact/technical-services or 1-315-986-8090.

An E2 Fault is declared when the thermistor to the In-line Heater is reporting an erroneously low reading of < 10˚C.

Note: An E2 fault can be caused by cold buffer running through the instrument. If you store your buffer solutions in the refrigerator, bring them to room temperature before starting a run.
 
The fault message on the Touch Screen Display will indicate that the In-line Heater is disabled.  The In-line Heater operates during IDF water rinses and 95% ethanol pre-heat when filling the SDF bags before the precipitation phase.  The run will be automatically aborted with this fault.   If a temperature of 8˚C is seen for the in-line heater, this is likely a false reading and would indicate a disconnect in the Thermistor circuit. Turn the power off and open the electrical enclosure. Identify the “MUX-T” circuit board.  Locate the 3-pin connector labeled “J10” and be sure that this is securely connected to its location on the MUX-T board and that the red and white wires do not pull out of the connector easily.  If the J10 connector is loose, connect it securely and see if this resolves the problem.  
 
If the problem continues the TDF98 TDF Thermistor Assembly within the In-Line Heater will need to be replaced.  If the problem persists, there may be an issue with the MUX-T board.
 
Note: If known, note the temperature of the In-Line Heater when this fault occurred. Depending on several factors, the SDF results from this run may not be accurate.  Some samples may be more sensitive to lower temperature alcohol during the precipitation phase than others.  If this fault occurred late in the 95% alcohol pre-heat / delivery, the alcohol may have nearly reached the desired temperature.

For replacement of the TDF98 TDF Thermistor Assembly see Service Procedure TS031 Thermistor Assembly Replacement to replace the thermistor.

For further assistance, contact ANKOM Technology at https://www.ankom.com/contact/technical-services or 1-315-986-8090.
 

To get this fault, the Temperature Sensor in one or more of the paddle heaters are reporting high temperatures. This could occur at different times under specific conditions.

• During the Amylase phase or when the instrument is idle if a temperature of over 105^°C is reported.
• During the cooling cycle (after the Amylase phase) if after 20 minutes the bags have failed to drop in temperature to 62°C.
• During the AMG or Protease phase if a temperature of over 70°C is reported.

Should any one of these conditions be met during a run, the run will be aborted. The E3 error message on the Touch Screen Display will indicate which station(s) are reporting the over-temp fault. This may be an actual over-temp condition, or it may be erroneous due to a connectivity issue. Either way the heaters will be shut down by the program and the run is aborted. If this were to occur during an SDF precipitation, the heaters will be shut down, but the SDF precipitation will continue as normal.

To determine if the over-temp condition is real and the result of a heater that is failing to cycle or turn off, run the following test. Turn the power off to the instrument and allow the paddles to cool to room temperature. Now turn the power back on and from the Touch Screen Display, select Diagnostics. Press Temperatures to monitor the reported temperatures. At the same time, by touch, check the temperature of each paddle, especially the one that faulted earlier. If the suspect paddle begins getting warm, there is a problem with the paddle heater control. Turn the power off. This will require the replacement of the I/O-2 circuit board (8300). Contact ANKOM Technology for further assistance https://www.ankom.com/contact/technical-services or 1-315-986-8090.

If the previous test does not reveal a problem with the paddle(s) heating unregulated, take the following steps to identify the problem. Turn the TDF instrument power off and then back on.  From the Select a Function screen, press Diagnostics and select Temperatures. See if the suspect paddle heater is again reporting an abnormally high (false) reading while sitting at room temperature. If so, there is a fault in one of the temperature sensors and the Paddle Bar Assembly needs to be replaced (TDF23) or with the newer style Paddle Bar Assembly, the individual paddles (TDF48) can be replaced. (Contact ANKOM to verify which paddle assembly you have).

If the readings appear normal and the E3 fault does not recur, run a brief test. Again, using the Diagnostics mode, select Heater Test, press Paddle Heater Power Enable. Having done so, turn on all six paddle heaters.  Monitor the temperatures closely to see if the E3 fault returns during heating of the paddles. Do not leave the instrument at this time as the heaters will stay on potentially overheating them if not controlled. Turn the heaters off when they reach 90°C.  Allow the paddles to cool back to ambient temperature. If during the heating or cooling, the fault recurs, the Paddle Bar will need to be replaced or with the newer style Paddle Bar Assembly the individual paddles (TDF48) can be replaced (Contact ANKOM to verify which paddle assembly you have). 

If the fault does not happen again, leave power on to the instrument overnight while in the Select a Function screen.  The program is always monitoring temperatures when power is on and if the fault occurs again during this time, the Paddle Bar will need to be replaced or with the newer style Paddle Bar Assembly the individual paddles (TDF48) can be replaced (Contact ANKOM to verify which paddle assembly you have). If the fault does not recur during any of these tests, it is possible that a voltage fluctuation or static discharge near the sensor may have caused this fault.  The instrument may be put back into use.  Keep record of any further E3 faults and if any do occur again, replace the Paddle Bar Assembly.

Refer to TDF Service Procedure #21 for replacement of the Paddle Bar Assembly (TDF23).  This will require calibration of the temperature sensors and proper setting of the mixing stroke.  If the problem persists or any questions arise, contact ANKOM Technology for further assistance https://www.ankom.com/contact/technical-services or 1-315-986-8090.
 

I am getting an E4 In-Line Heater Overtemp fault; what should I do?
 
This fault occurs when the Temperature Sensor in the In-Line Heater reports a temperature exceeding 135˚C.   The E4 error message on the Touch Screen Display will indicate the temperature at the time of the fault. When this fault occurs the instrument automatically aborts the current run.
 
The In-Line Heater operates during IDF water rinses and when filling the SDF bags with 95% alcohol.  Possible causes for the E4 fault include:
 
1.        Empty chemical container during In-line heater operation (causing an actual overheat)
This fault can occur when water or alcohol containers are not filled adequately before the run and if they empty during the In-Line Heater operation. This may cause the In-Line Heater to exceed 135˚C in which case the heater will be disabled and the run aborted. If this happens one will likely see an E14 Empty Chemical Container fault as well.  Were this to happen, the instrument is performing as intended to prevent uncontrolled heating of the In-Line Heater. Be sure to start a run with containers filled to the minimum level line.
 
2.        A short in the thermistor wiring (causing a false high reading) or a malfunctioning MUX-T circuit board (causing a false high reading)
A reading of 161˚C is reason to suspect a fault in the thermistor wiring. If this is the case, confirm that the In-line heater is not actually at this temperature. Turn the power off to the instrument and allow the In-Line Heater to cool to room temperature. Remove the clear back panel from the rear of the instrument. Turn the instrument back on and from the Select a Function screen press Diagnostics and then select Temperatures. Check to see that the In-Line Heater temperature sensor is reporting a temperature in line with the actual room temperature. If an IR thermometer is available this can be used to confirm that the In-line heater reading reflects its actual temperature.  Be cautious when doing so as it could be getting very hot if there is a problem with the heater control. If the In-Line Heater is physically cool while a high temperature is reported on the display, then there is a problem with either the thermistor (TDF98 temperature sensor) or MUX-T circuit board (TDF111). Turn power off to the instrument.
 
3.        Malfunctioning Heater Relay or I/O-2 Circuit board (causing an actual overheat)
If the In-Line Heater is actually overheating and this is confirmed both by sensing the heat coming off the heater and by a steady rise in temperature on the In-Line Heater Temperatures display – turn power off and unplug the instrument. In this case there would be a problem with either the Heater Relay (6032) or the I/O-2 Circuit board (8300). If this is the cause of the E4, inspect the in-line heater assembly for damage (i.e., severe discoloration of the brass tubes or coiled brass tubes now loose from the heater core).  If damage is found, replace the entire In-Line Heater assembly.
 
For replacement parts and further assistance, contact ANKOM Technology at https://www.ankom.com/contact/technical-services or 1-315-986-8090. 
To replace an In-Line Heater, click the In-Line Heater Replacement service procedure link shown here.
 

To get this fault, the temperature sensor in one or more of the paddle heaters is reporting low temperatures. This could occur at different times under specific conditions.

• During paddle pre-heat if any one of the paddles does not reach 50°C in 5 minutes.
• During the Amylase phase if any one of the paddle sensors does not reach 90°C in 25 minutes.  Also applies to Trizma phase in the AOAC2009.01/2011.25 methods.
• During the AMG or Protease phase if any of the paddle sensors fail to maintain at least 45°C (temperature range is 60°C +10/-15°C). Also applies to Protease phase during AOAC2009.01/2011.25 methods.

Should any one of these conditions be met during a run, the suspect heater/station will be shut down and stop heating.  The run will otherwise continue as normal. The E5 error message on the Touch Screen Display will indicate which station(s) are reporting the under-temp fault and what the temperature was at the time of the fault. This may be an actual under-temp condition, or it may be erroneous due to a connectivity issue.  Either way the affected heater will be shut down by the program and the run will continue. If this were to occur during the IDF phase, with the heater being shut down, digestion will likely not occur as normal and the test results will not be reliable for this station.

Below is a list of potential causes of the E5 fault:

1. Bag breakage: Confirm that an IDF or Flow-thru bag did not break during one of the heating periods. If a bag were to leak near Clamp Bar B and if fluids were to get on one of the paddles, the liquid evaporation will drop the paddle temperature and possibly cause this fault.  If this has occurred, the instrument is sensing a real drop in temperature at the wetted paddle and no repair to the instrument is needed. Clean the area from the leaked bag. Test results from a broken bag should be disregarded.
2. Greater volume in the IDF or Flow-thru bags: Confirm that the volume calibration is accurate. Rerun the volume calibration as described in the operator's manual (QC/Calibration Checks section). If, for example, the volume calibration is significantly off, there could be an average of 50mls of buffer delivered instead of the required 40mls. This would create a delay in reaching temperature.
3. Pre-heat of paddles has been by-passed: In Diagnostics there is the option under Service Mode to "Bypass Amylase Phase Initial Heat-up." If this has been inadvertently pressed (stating "YES") this will bypass the paddle preheat to 50°C and take longer to heat up once the Buffer/Amylase is added. Make sure this option says "NO." This feature is typically used only for demo purposes.
4. Clamp Bar A is left open: If for any reason Clamp Bar A was left open during the Amylase phase heat up, there will be more evaporative cooling which will require more time to heat the bags. NOTE: normally Clamp Bar A will close automatically after the final delivery to the IDF positions, but it can be re-opened.
5. Front cover left open or localized draft: Be sure to use the front safety cover, especially during the Amylase phase. If this cover is left open, and/or if there is a nearby A/C vent creating a draft toward the front of the instrument the Amylase phase heat-up will take longer. Also, if the TDF instrument is located inside a hood, turn off the air flow to the hood during the Amylase phase heat-up. The continued upward airflow will create a significant delay in reaching temperature. Addressing these may resolve the problem.
6. Alcohol - Buffer containers connected incorrectly: Ensure that the containers are connected correctly to the color-coded supply lines on the right side of the instrument. Confirm that alcohol did not inadvertently get put in the buffer container, and that buffer was not put in the alcohol containers. If either of these things happen, and alcohol gets dispensed to the IDF bags at the start of the Amylase phase, the alcohol will boil at a much lower temperature and not reach the target temperature of 97°C.

If the E5 fault was not the result of one of the above issues, run a brief test to see if all the paddle heaters are heating normally. Allow the paddles to return to room temperature. Obtain a stopwatch and 6 Flow-thru bags (used bags will work for this test). Use a heat sealer to seal the bottom edge of the bags. This will make removal later easier. Install these in the IDF positions. Connect the TDF70 Flush Tubing assembly to the HCL, 3 enzymes, buffer, water, and 2 alcohol connection points on either side of the instrument. Connect the middle connector to a filled Distilled Water container. From the Select a Function screen select a 991.43 TDF run. Answer all the prompts affirmative and press START. Press the "Display Temps/Pressures" button to monitor the temperature readings and watch the time that they reach 50°C. They should reach this temperature in about 2 to 2.5 minutes depending on the ambient starting temperature. Note the amount of time required. Reset your timer. As soon as Amylase and Buffer are delivered, the mixing will start.  Restart your timer now. Use the chart below to record every 2.5 minutes, the time it takes for each paddle to reach 90°C. T₀ is the end of the fluid delivery to the bags and start of mixing. Once they all reach 90°C, press ABORT to end the run and allow the bags to cool before removal.


 
If the suspect paddle heater takes significantly longer to heat than the others or fails to heat up, there is a problem with either the heater within the Paddle Bar Assembly or with the I/O2 circuit board. If the Paddles heat to the touch but one of the sensors shows an erroneous low reading, there is a problem with the sensor, and the Paddle Bar will need to be replaced or with the newer style Paddle Bar Assembly the individual paddles (TDF48) can be replaced (Contact ANKOM to verify which paddle assembly you have). 
 
For further assistance, contact ANKOM Technology at https://www.ankom.com/contact/technical-services or 1-315-986-8090.  Follow TDF Service Procedure #TS021 for replacement instructions of the Paddle Bar assembly (TDF23).

If one of the paddles fails to heat normally, and to rule out a problem with the I/O-2 circuit board, remove the back and top panel of the Electrical Enclosure so that the I/O-2 circuit board can be seen. Do not touch wiring. Perform this simple test:
1. In Diagnostics, select Heater Test.
2. Select Paddle Heater Power Enable.
3. Check that LED #2 lights on the I/O-2 board in the electrical enclosure.
4. Leaving the above enabled, select Paddle #1, and inspect the LED (refer to chart below for each LED).
5. Repeat for each paddle heater. The following is a list of the paddles and the corresponding LED #:

• Paddle Heater Power Enable - LED 2
• Paddle 1 - LED 15
• Paddle 2 - LED 12
• Paddle 3 - LED 9
• Paddle 4 - LED 7
• Paddle 5 - LED 4
• Paddle 6 - LED 1

Turn heaters off after each test. If the appropriate LED is not lighting up, the problem may be with either the power supply or the I/O2 circuit board. 
To test the power coming from the power supply to the individual paddle heater you will need a voltmeter set to DC. Connect the red lead on the voltmeter to the #20 position on the 24-volt DIN rail.  Now turn all the heaters on and test voltage as follows. On the back of the I/O2 circuit board are two 10-pin connectors labeled CN-3 and CN-4.  To test the power to each paddle, touch the black lead of the voltmeter to the following contact. 

• Paddle 1 - CN4, #2 position (wire label 53)
• Paddle 2 - CN4, #5 position (wire label 50)
• Paddle 3 - CN4, #8 position (wire label 47)
• Paddle 4 - CN3, #2 position (wire label 45)
• Paddle 5 - CN3, #5 position (wire label 42)
• Paddle 6 - CN3, #8 position (wire label 39)

 
You should detect 25.0 volts +/- 0.2 volts at each circuit.  If voltage is low there may a problem with the power supply or voltage adjustment.  For details on replacing TDF110 Power Supply B see Service Procedure #TS032 Power Supply B Replacement (TDF110).   
 
If all the LEDs are lighting up as normal and voltage is normal, the problem is with a paddle heater within the Paddle Bar Assembly.  Follow Service Procedure #TS021 for replacement instructions of the Paddle Bar assembly (TDF23) or with the newer style Paddle Bar Assembly the individual paddles (TDF48) can be replaced (Contact ANKOM to verify which paddle assembly you have).
 
For further assistance, contact ANKOM Technology at https://www.ankom.com/contact/technical-services or 1-315-986-8090. 
 

Cause: This fault occurs when the Temperature Sensor in the In-Line Heater reports that the In-Line Heater is not reaching the required 110˚C within the 90 seconds allotted during an IDF water rinse.  The fault message on the Touch Screen Display will indicate that the In-line Heater is disabled.  The operator will be able to select OK and continue the run, but the cause should be investigated later.
 
The In-line Heater operates during IDF water rinses to facilitate rinsing of the IDF bag and to ensure that solutions when deposited into the SDF bag are nominally the same temperature as the pre-heated alcohol.  The run in progress will continue despite this fault.  If known, note the temperature of the In-Line Heater when this fault occurred.

Note:  This fault can be falsely declared if the Buffer or Water are kept in a refrigerator.  The Buffer and water should be allowed to warm prior to the start of a routine.
 
Result: Typically, the test results from this run would not be affected by the cooler water rinse temperatures. Be sure that the sample in the IDF bag was rinsed down into the filter area of the bag and observe the precipitation phase for SDF.  If any anomalies are noted pay particular attention to the test results as it is possible results for certain sample types may be more sensitive to lower temperature during the precipitation phase than others.  It may be advisable to repeat the run after the problem is corrected. 
 
Troubleshooting: After the assay with the fault is complete, turn the power off to the instrument and turn it back on to clear the fault. Test the In-line heater under Diagnostics / Heater Test.  With a timer handy, observe how quickly the in-line heater heats up. Start the timer and immediately turn the In-line heater on and watch for it to get up to 70˚C.  It should reach 70˚C in 40-45 seconds. Turn it off as soon as 70˚C is reached. Confirm with an IR thermometer that the temperature reading on the touch screen display matches the actual temperature of the In-line heater based on the reading taken with the IR thermometer.  The two readings should be ± 5˚C of each other.   
 
Note: If the In-line heater temperature appears to be frozen at 8˚C, there is likely a problem with a disconnected Thermistor wire at the MUX-T board or a lack of continuity in the Thermistor wiring.
 
If this fault is the result of a false low temperature reading, or if you need any further assistance, contact ANKOM Technology at https://www.ankom.com/contact/technical-services or 1-315-986-8090. 
 
For replacement of an in-line heater, click the In-Line Heater Replacement service procedure link shown below.
 

Cause: This fault occurs when the Temperature Sensor to the In-Line Heater reports that the In-Line Heater is not reaching the required 80˚C within the 90 seconds allotted during an EtOH95 delivery.  The fault message on the Touch Screen Display will indicate that the In-line Heater is disabled.  The operator will be able to select OK and continue the run, but the cause should be investigated later.
 
Result: The In-line Heater operates during the EtOH95 delivery to the SDF bags.  The run in-progress will continue despite this fault.  If known, note the temperature of the In-Line Heater when this fault occurred. Typically, the test results from this run would not be affected by the cooler ethanol temperatures. Observe the precipitation phase for SDF.  If any anomalies are noted pay particular attention to the test results as it is possible results for certain sample types may be more sensitive to lower temperature during the precipitation phase than others.  It may be advisable to repeat the run after the problem is corrected. 
 
Trouble: After the assay with the fault is complete, turn the power off to the instrument and turn it back on to clear the fault. Test the In-line heater under Diagnostics / Heater Test.  With a timer handy, observe how quickly the in-line heater heats up. Start the timer and immediately turn the In-line heater on and watch for it to get up to 70˚C.  It should reach 70˚C in 40-45 seconds. Turn it off as soon as 70˚C is reached. Confirm with an IR thermometer that the temperature reading on the touch screen display matches the actual temperature of the In-line heater based on the reading taken with the IR thermometer.  The two readings should be ± 5˚C of each other.
 
Note: If the In-line heater temperature appears to be frozen at 8˚C, there is likely a problem with a disconnected Thermistor wire at the MUX-T board or a lack of continuity in the Thermistor wiring.
 
If this fault is the result of a false low temperature reading, or if you need any further assistance, contact ANKOM Technology at https://www.ankom.com/contact/technical-services or 1-315-986-8090. 
 
For replacement of an in-line heater, click the In-Line Heater Replacement service procedure link shown below.
 

This fault occurs when the Temperature Sensor reports a high temperature while the In-Line Heater is attempting to cool within the required timeframe.  Cooling is facilitated by flushing water through the lines of the In-line heater.  
 
The first time this occurs during a run, the operator will have the option to “CONTINUE” or “ABORT”.  If “CONTINUE” is selected the pump will resume flushing water through the in-line heater to bring the temperature down. If high temperature is reported again, the run will be aborted, and an E9 In-line Heater Overtemp Fault will be displayed.  
 
Possible causes for the E9 fault include:
 
1.        Empty chemical container during In-line heater cool down cycle (causing a cool-down failure)  
2.        A short in the thermistor wiring or a malfunctioning MUX-T circuit board  
3.        Malfunctioning Heater Relay or I/O-2 Circuit board  
For replacement parts and further assistance, contact ANKOM Technology at https://www.ankom.com/contact/technical-services or 1-315-986-8090.  For replacement of an in-line heater or MUX-T board, click the service procedure link shown below.
 

An E10 fault is the result of one or more of the six pressure sensors reading a pressure 20 psi above the baseline pressure during the buffer delivery to the IDF bags or during the EtOH95 delivery to the SDF bags.  This fault will abort the run.  The fault screen will identify which line or lines are showing the high pressure.
 
The two main reasons for this fault are:

1. The pressure in the line is in fact high when the pump is delivering Buffer to IDF or EtOH95 to SDF, due to an obstruction or blockage in the fluid path.
2. One of the pressure sensors could be faulty.

 
To analyze this problem, do the following:

1. Check the pressure reading displayed when the E10 fault code appeared.  If the reading was about 37 psi, this could indicate a failed pressure sensor because 37 psi is the maximum value these sensors will read and can be caused by an open circuit/disconnected wire.
2. Confirm the baseline pressure readings from the six-line pressure sensors.  Go to Diagnostics / Pressures and identify the readings from Lines 1 – 6.  If you are at sea level the pressure should be approximately 14.2 – 15.2 psi depending on weather.  The readings will vary significantly from a 14.7 psi base line due to elevation (i.e., in Denver, CO the base line pressure will be about 12.0 psi).  Are the six pressure sensors reading within 0.5 psi of each other and in line with your local elevation? 

 
If one or more of the Line pressures is significantly out of line with the others, then the TDF Pressure Sensor Assembly (TDF19) will need to be replaced.
 
If the six line pressures appear normal when the instrument is idle, to test further and view the dynamic pressures within each line during operation, run the following test:
 
E10 Fault Test:

1. Obtain 6 Flow-thru bags and heat seal the bottom edges.
2. Install these Flow-thru bags in the IDF positions 1-6.
3. Connect the TDF70 Flush Tube Assembly to the 8 fluid ports or tubes.
4. Connect the middle connector of the TDF70 Flush Tube Assembly to the Deionized Water container.
5. On the Select-a-function screen select AOAC991.43 TDF.
6. Start the 991.43 procedure by following the prompts on screen.  There is NO need for samples, for clamp bars C&D, or for SDF bags.
7. The paddles will preheat to 50°C and then fluid delivery will start to the Flow-thru bags.
8. As soon as delivery begins to the Flow-thru bags press the button “View Temps/Pressures”, then press “Pressures”. Bag filling takes only about 1 minute, so you need to do this rather quickly.
9. Observe the pressure readings during delivery at lines 1-6 and record these. 
10. When the delivery is finished you can abort the run.

 
What do the dynamic pressures reach during the delivery to the Flow-thru bags?
Typically, the six-line pressures will climb 1.5 – 3.5 psi above the baseline reading.  So, if your baseline reading is 14.5 psi, pressure readings of 16.0 – 18.0 psi would be normal when the pump is running.
 
The E10 Fault is only triggered if a line pressure reaches 20.0 psi above the baseline reading.  In the case of a baseline reading of 14.5 psi, the dynamic pressure would need to reach 34.5 psi.
 
If the pressures are climbing significantly above 18.0 psi, investigate further the source of a blockage or obstruction in the fluid path.
 
Here are the places that could be causing a blockage and should be inspected:

1. The Pinch Valve tubing (TDF71).  These should be changed annually to prevent sticking.
2. The Fill Nozzles (TDF79) or Spray Tips (TDF44).  Spray tips can be cleaned with the spray tip cleaning tool or replaced.  The entire fill nozzle can also be replaced as needed.
3. The silicone tubing within the Tubing Support Panel (TDF30).  Due to the complexity of this tubing assembly, it may be necessary to replace the entire component if a blockage is suspected within this part.
4. The Silicone Tubing 1/16’ x 3/16” (TDF77) that connects the Tubing Support Panel to the Fill nozzle.
5. The Barbed Tube Fittings (TDF104) that attach to the vertical gusset panels near the pinch valves.
6. The Brass in-line heater coils on the In-Line Heater Core (TDF120).  If a blockage or corrosion cannot be flushed out of the brass tubing this component will need to be replaced.
7. The Pinch Valve IDF-SDF (TDF29) may be stuck closed due to a problem with the pneumatic piston.  This can be cycled and inspected after removing the tubing to confirm operation by using the Diagnostics screen / Valve Test and cycling the IDF and SDF Inlet valves.

 
If all the parts in the list above were to be replaced the entire flow path of the fluid from the pressure sensor assembly to the bags will have been replaced.  This would address an actual blockage creating the E10 fault.  If any or all these steps are taken it is advisable to rerun the E10 fault test described above.
 
For further assistance, contact ANKOM Technology at https://www.ankom.com/contact/technical-services or 1-315-986-8090.
 

An E11 fault is the result of the high-pressure sensor reading input pressure below 35 psi.  N2 Input pressure is always being monitored and the fault could occur at any point during a run. The fault will interrupt the run and wait for the operator to correct the problem. This problem can result from one of the following causes:

Refer to TDF Service Procedure TS001, to diagnose a tubing pressure failure.

The Touch Screen Display will give you the option to Retry, Override, or Abort the run.  Retry will simply try the delivery again; Override will ignore the fault for the remainder of the run; and Abort will stop the run entirely.

The most likely cause of an E14 fault is an empty chemical container.  The fault screen will identify the container that was being drawn from when the error occurred.  The vacuum sensor is designed to monitor vacuum pressure on the supply lines when they are being drawn from.  When there is little or no vacuum on the line, this fault is declared.  Refill the appropriate container as needed and select “Retry”.

Another potential cause of an E14 fault is a leak in any one of the supply tubes or fittings that would allow air to get pulled into a supply line. To test for this, under “Diagnostics”, run a “Motor Test" and then“Set Valves”.  Open the supply line valve that generated the fault and open the output Waste valve.  Set the volume to 40 mls and press “GO”.  Observe the supply lines at the rear of the instrument or tilt the instrument forward using the pivot mechanism to see the supply lines. Look for any air being drawn in at a hole in the tubing or at a crack in a fitting.  If this is observed, tubing or fittings may need to be replaced.  For further assistance contact ANKOM Technology at https://www.ankom.com/contact/technical-services or 1-315-986-8090.

Another possible cause of an E14 fault is an interruption in the Pump Motor Controller (PMC) operation.  If the PMC hesitates or stops because it has overheated or because the electronics on the circuit board have been compromised, the pause in operation will cause a drop in vacuum pressure and trigger an E14 fault.   This is possible even if the pause in operation were only for a second or two.  You should be able to retry and continue without a problem if that is the case; however, perform further testing after the run is complete.  After the run, perform a “Motor Test” under “Diagnostics” after first setting valves to Water Supply open and Waste open with all other valves closed. Set the volume to    250 mls – this will simulate the duration of operation for a 95% alcohol delivery. Fill and plug in the water container.

Press “GO” and listen for the Pump Motor operation.  Be attentive to any pause in the pump’s operation. The pump should run continuously for 6.5 – 8.5 minutes, depending on the volume calibration setting for your specific instrument. Stay with the instrument for the entire delivery.  Refill the water container when it nears empty, as it will draw more than 1 liter of water (250mls x 6).    If no pause in operation is noticed, allow the instrument to sit idle for 10 minutes and repeat the test.  If a hesitation or pause in operation is witnessed during the lengthy pump operation time the Pump Motor Controller (8301) will need to be replaced.

For further assistance contact ANKOM Technology at https://www.ankom.com/contact/technical-services or 1-315-986-8090.
 

The Touch Screen Display will give you the option to retry, override, or abort the run. 

• Retry will simply try the delivery again.
• Override will ignore the fault for the remainder of the run.
• Abort will stop the run entirely.

 
To analyze the problem, do the following:
When the instrument is not in the process of running an analysis, an E15 fault can be investigated by performing a Motor Test under Diagnostics.  From the Motor Test screen, press Set Valves and select the valve that caused the fault.  If this is not known, test each supply valve. Open only one supply valve at a time and close all others.  Open the Waste output valve and close the IDF and SDF output valves.  Be sure that there is adequate fluid in the reservoir that is being tested. Change the delivery volume to 20 ml and press GO.  Watch the vacuum reading on the screen.  Typical readings are between 0.7 – 1.5 psi.  A reading above 3 psi will generate an E15 fault (during a run).  If one of the supply lines shows a high reading, you have isolated the supply line that is causing the problem.
              
Possible causes of an E15 fault include:
1) Clogged Filter
The most likely cause of an E15 fault at the Water, Buffer, EtOH78, EtOH95 or HCl lines is a clogged filter. Severely clogged filters may reduce delivery volumes and affect results. The filters are at the end of the draw tubes inside of the containers.  These should be checked monthly and cleaned as needed. The filters can usually be cleaned with a soft bristle brush under running water. If they can't be cleaned, there are two spares of the plastic type container filters (used for water, alcohols, and HCl/Acetic Acid) and one spare of the metal screen filters (used for buffer) included with the instrument on original purchase. Additional ones may be ordered as part 8202 (plastic filter) and part 8203 (metal screen filter).    
 
2) Quick Disconnect Fitting not fully locked in place
A quick disconnect fitting functions as a valve that closes when disconnected or not fully connected. Quick disconnect fittings are found at the following reservoirs: enzymes, water, and alcohols.  Check to make sure that they cannot be pulled out without pressing the release button. If they can be pulled out without use of the release button they are not fully engaged, and fluids will not be drawn. Push them into the adjoining fitting until you hear them snap in place. Confirm that they cannot be pulled out. It is also possible that the fittings may need to be replaced – see part 8193 (coupling body) and 8194 (coupling insert) on the ANKOM website.
 
3) Worn tubing in the Enzyme Pinch Valve
An E15 fault at one of the Enzyme lines (Amylase, Protease, or AMG) could be due to worn tubing in the Enzyme Pinch Valve. As the tubing in the Enzyme Pinch Valve ages, it may become stuck closed preventing the flow of enzymes. Review and follow Service Procedure TS004, first replacing the tubing within the pinch valve.  These tubes should be replaced annually. Spare pinch valve tubing comes with the TDF instrument upon original purchase; additional ones can be ordered as part TDF71 Pinch Valve Tubing Set (21 pcs). Also inspect the supply lines to see if there is a clog or pinch in the line. These also may require replacement.  A set of all enzyme tubes can be ordered as part TDF67 Enzyme Tubing Replacement Kit.  If the problem persists, contact ANKOM Technology at https://www.ankom.com/contact/technical-services or 1-315-986-8090 for further assistance.
 
4) Pinched or plugged supply line or a faulty supply valve
Other possible causes for an E15 fault are a pinched or plugged supply line or a faulty supply valve.  Inspect the supply line in question to see if such a problem can be seen visually.  After having cleaned and/or replaced the filter and having checked for fully engaged disconnect fittings, if the problem persists, contact ANKOM Technology at https://www.ankom.com/contact/technical-services or 1-315-986-8090 for further assistance.
 

For instruments with version 3.78 or newer software perform the following diagnostics routine:

 

1.	Run Line Charge routine two times with “All” selected with the flush tube assembly installed using deionized water.  This will prime the lines in the instrument.
2.	Set up Motor test with the Flush Tubes still connected.
	a. Set all Supply Valves to Closed b. Set Speed to 240 RPM c. Leave Amount set to 10 ml d. Press Start
3.	At the start of the routine, in the upper right corner of the screen you should see a number approximately 14.  Part way through the routine you should see it say “Plugged”.  This is normal for this stage of the test.
4.	Below the word “Plugged” you will see a set of numbers that will climb throughout the routine and should end in the range of 11 or higher.  Any number below 11 indicate a leaky valve.  In most cases, the valve causing the issue will be the buffer valve.
5.	Refer to Service Procedure TS-017 for maintenance instructions for the valve.  If the valve body is pitted, especially around the hole in the center, it will be necessary to replace the valve body using the TDF25.5 kit.  Follow Service Procedure TS046 for installation instructions.